Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1199079..1199806 Replicon chromosome
Accession NZ_CP058217
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag HW372_RS05760 Protein ID WP_000547555.1
Coordinates 1199079..1199390 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag HW372_RS05765 Protein ID WP_000126293.1
Coordinates 1199387..1199806 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW372_RS05730 (1194220) 1194220..1195929 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
HW372_RS05735 (1195939) 1195939..1196481 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
HW372_RS05740 (1196481) 1196481..1197248 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
HW372_RS05745 (1197245) 1197245..1197655 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
HW372_RS05750 (1197648) 1197648..1198118 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
HW372_RS05755 (1198161) 1198161..1198916 + 756 WP_001305268.1 hypothetical protein -
HW372_RS05760 (1199079) 1199079..1199390 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
HW372_RS05765 (1199387) 1199387..1199806 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
HW372_RS05770 (1200027) 1200027..1200326 - 300 WP_001704025.1 DUF4259 domain-containing protein -
HW372_RS05775 (1200711) 1200711..1201238 + 528 WP_001078777.1 electron transport protein HydN -
HW372_RS05780 (1201391) 1201391..1203643 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T166177 WP_000547555.1 NZ_CP058217:1199079-1199390 [Escherichia coli Nissle 1917]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T166177 NZ_CP074052:c3759207-3758905 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCTGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT166177 WP_000126293.1 NZ_CP058217:1199387-1199806 [Escherichia coli Nissle 1917]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT166177 NZ_CP074052:c3758869-3758528 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References