Detailed information of TA system TA166174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 621471..622270
• Replicon: chromosome
• Accession: NZ_CP058217
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: HW372_RS03050
• Protein ID: WP_000347252.1
• Coordinates: 621471..621935 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: HW372_RS03055
• Protein ID: WP_001296435.1
• Coordinates: 621935..622270 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW372_RS03020 (616472)	616472..616906	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HW372_RS03025 (616924)	616924..617802	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
HW372_RS03030 (617792)	617792..618571	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HW372_RS03035 (618582)	618582..619055	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HW372_RS03040 (619078)	619078..620358	-	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HW372_RS03045 (620607)	620607..621416	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HW372_RS03050 (621471)	621471..621935	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HW372_RS03055 (621935)	621935..622270	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HW372_RS03060 (622419)	622419..623990	-	1572	WP_001273940.1	galactarate dehydratase	-
HW372_RS03065 (624365)	624365..625699	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
HW372_RS03070 (625715)	625715..626485	+	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T166174 WP_000347252.1 NZ_CP058217:c621935-621471 [Escherichia coli Nissle 1917]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T166174 NZ_CP074052:c3109318-3108935 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT166174 WP_001296435.1 NZ_CP058217:c622270-621935 [Escherichia coli Nissle 1917]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT166174 NZ_CP074052:c3109779-3109399 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5