Detailed information of TA system TA166151

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 33504..33757
• Replicon: plasmid pRHB24-C12_3
• Accession: NZ_CP058211
• Organism: Escherichia marmotae strain RHB24-C12

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HVY12_RS23735
• Protein ID: WP_001312851.1
• Coordinates: 33504..33653 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	33698..33757 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY12_RS23710	28995..29102	+	108	WP_157924485.1	hypothetical protein	-
HVY12_RS23720	31812..32669	-	858	WP_137585087.1	incFII family plasmid replication initiator RepA	-
HVY12_RS23725	32662..32736	-	75	WP_001375168.1	RepA leader peptide Tap	-
HVY12_RS23730	32960..33220	-	261	WP_000083819.1	replication regulatory protein RepA	-
HVY12_RS23735	33504..33653	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	33698..33757	+	60	NuclAT_0	-	Antitoxin
-	33698..33757	+	60	NuclAT_0	-	Antitoxin
-	33698..33757	+	60	NuclAT_0	-	Antitoxin
-	33698..33757	+	60	NuclAT_0	-	Antitoxin
HVY12_RS23740	34301..34531	-	231	WP_181238297.1	hypothetical protein	-
HVY12_RS23745	34700..35299	-	600	WP_181238298.1	hypothetical protein	-
HVY12_RS23750	35671..35865	+	195	WP_024234536.1	hypothetical protein	-
HVY12_RS23755	36019..36579	-	561	WP_000139377.1	fertility inhibition protein FinO	-
HVY12_RS23760	36653..37402	-	750	WP_181238299.1	type-F conjugative transfer system pilin acetylase TraX	-
HVY12_RS23765	37410..37631	-	222	WP_181238300.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..95282	95282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T166151 WP_001312851.1 NZ_CP058211:c33653-33504 [Escherichia marmotae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T166151 NZ_CP074047:2609512-2609619 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT166151 NZ_CP058211:33698-33757 [Escherichia marmotae]
AACGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin