Detailed information of TA system TA166145

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72806..73070
• Replicon: plasmid pRHB24-C12_2
• Accession: NZ_CP058210
• Organism: Escherichia marmotae strain RHB24-C12

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HVY12_RS23310
• Protein ID: WP_001331364.1
• Coordinates: 72918..73070 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	72806..72863 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY12_RS23295	68045..70336	-	2292	WP_181238237.1	conjugal transfer protein TrbC	-
HVY12_RS23300	70329..71399	-	1071	WP_181238238.1	IncI1-type conjugal transfer protein TrbB	-
HVY12_RS23305	71418..72626	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	72806..72863	-	58	NuclAT_0	-	Antitoxin
-	72806..72863	-	58	NuclAT_0	-	Antitoxin
-	72806..72863	-	58	NuclAT_0	-	Antitoxin
-	72806..72863	-	58	NuclAT_0	-	Antitoxin
HVY12_RS23310	72918..73070	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
HVY12_RS23315	73142..73393	-	252	WP_160529436.1	hypothetical protein	-
HVY12_RS23320	74052..74228	-	177	WP_001054897.1	hypothetical protein	-
HVY12_RS23325	74437..74646	-	210	WP_000416064.1	hemolysin expression modulator Hha	-
HVY12_RS23330	74744..75358	-	615	WP_000578646.1	plasmid IncI1-type surface exclusion protein ExcA	-
HVY12_RS23335	75434..77602	-	2169	WP_181238239.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeC / faeD / faeE / faeF / faeH / faeI / faeJ / paa	1..119877	119877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T166145 WP_001331364.1 NZ_CP058210:72918-73070 [Escherichia marmotae]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T166145 NZ_CP074047:1764467-1764718 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT166145 NZ_CP058210:c72863-72806 [Escherichia marmotae]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin