Detailed information of TA system TA166097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 73922..74649
• Replicon: plasmid pRHBSTW-00002_3
• Accession: NZ_CP058198
• Organism: Enterobacter roggenkampii strain RHBSTW-00002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HV027_RS23185
• Protein ID: WP_011251285.1
• Coordinates: 74338..74649 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV027_RS23180
• Protein ID: WP_011251286.1
• Coordinates: 73922..74341 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV027_RS23150	69300..70307	-	1008	WP_032413314.1	DUF1611 domain-containing protein	-
HV027_RS23155	70792..72000	+	1209	WP_015386429.1	IS256 family transposase	-
HV027_RS23160	72110..72580	-	471	WP_004883378.1	RES family NAD+ phosphorylase	-
HV027_RS23165	72577..73020	-	444	WP_032413443.1	DUF2384 domain-containing protein	-
HV027_RS23170	73121..73350	-	230	Protein_68	DUF4113 domain-containing protein	-
HV027_RS23175	73391..73708	+	318	Protein_69	transposase	-
HV027_RS23180	73922..74341	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HV027_RS23185	74338..74649	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV027_RS23190	74854..75914	-	1061	Protein_72	IS3 family transposase	-
HV027_RS23195	75964..76380	-	417	WP_057072957.1	type II toxin-antitoxin system VapC family toxin	-
HV027_RS23200	76377..76607	-	231	WP_057072956.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HV027_RS23210	78032..78388	+	357	Protein_76	hypothetical protein	-
HV027_RS23215	78558..78776	+	219	WP_016240352.1	type II toxin-antitoxin system antitoxin CcdA	-
HV027_RS23220	78778..79083	+	306	WP_045616569.1	type II toxin-antitoxin system toxin CcdB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108466	108466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 15324.38 Da
• Isoelectric Point: 5.5500

>T166097 WP_011251285.1 NZ_CP058198:c74649-74338 [Enterobacter roggenkampii]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 312 bp

>T166097 NZ_CP074033:1854772-1854990 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 20622.74 Da
• Isoelectric Point: 5.5500

>AT166097 WP_011251286.1 NZ_CP058198:c74341-73922 [Enterobacter roggenkampii]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 420 bp

>AT166097 NZ_CP074033:1854372-1854746 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1