Detailed information of TA system TA166045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 49057..49784
• Replicon: plasmid pRHBSTW-00040_3
• Accession: NZ_CP058177
• Organism: Enterobacter hormaechei strain RHBSTW-00040

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV043_RS23955
• Protein ID: WP_155024128.1
• Coordinates: 49057..49368 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV043_RS23960
• Protein ID: WP_155024127.1
• Coordinates: 49365..49784 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV043_RS23925	45686..45916	+	231	Protein_41	antirestriction protein	-
HV043_RS23930	45929..46471	+	543	WP_032267592.1	DNA repair protein RadC	-
HV043_RS23935	46482..46823	+	342	WP_181241123.1	type IV toxin-antitoxin system YeeU family antitoxin	-
HV043_RS23940	46844..47185	+	342	WP_032267594.1	TA system toxin CbtA family protein	-
HV043_RS23945	47436..47546	+	111	Protein_45	restriction endonuclease	-
HV043_RS23950	47610..48590	+	981	WP_000019450.1	IS5-like element ISKpn26 family transposase	-
HV043_RS23955	49057..49368	+	312	WP_155024128.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV043_RS23960	49365..49784	+	420	WP_155024127.1	helix-turn-helix domain-containing protein	Antitoxin
HV043_RS23965	49788..49991	-	204	WP_045898927.1	hypothetical protein	-
HV043_RS23970	50271..51275	-	1005	WP_022542389.1	IS110-like element IS4321 family transposase	-
HV043_RS23975	51375..52079	+	705	Protein_51	IS6-like element IS26 family transposase	-
HV043_RS23980	52308..53846	-	1539	WP_155024093.1	IS66-like element ISKpn24 family transposase	-
HV043_RS23985	53895..54242	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
HV043_RS23990	54239..54643	-	405	WP_003031976.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	ugd / wbtL	1..60669	60669
	-	inside	IScluster/Tn	-	-	38045..54643	16598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.08 Da
• Isoelectric Point: 9.8988

>T166045 WP_155024128.1 NZ_CP058177:49057-49368 [Enterobacter hormaechei]
MHVISKEPFEEAAKQYPNNSLAVRALYRLVRETDFSSPAEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T166045 NZ_CP074028:579753-580040 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15489.65 Da
• Isoelectric Point: 4.3573

>AT166045 WP_155024127.1 NZ_CP058177:49365-49784 [Enterobacter hormaechei]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAIELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEMPV
GIALLRTLIDQYRLSYSDLKEEIGSKSLVSQILSGQRSLTIMHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT166045 NZ_CP074028:579514-579753 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA