Detailed information of TA system TA166017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 28762..29492
• Replicon: chromosome
• Accession: NZ_CP058172
• Organism: Enterobacter hormaechei strain RHBSTW-00056

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155SSD8
• Locus tag: HV047_RS00145
• Protein ID: WP_022646552.1
• Coordinates: 28762..29076 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV047_RS00150
• Protein ID: WP_023305145.1
• Coordinates: 29073..29492 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV047_RS00120	24843..25928	+	1086	Protein_23	cellulase family glycosylhydrolase	-
HV047_RS00125	25834..27018	-	1185	WP_023298963.1	multidrug efflux MFS transporter EmrD	-
HV047_RS00130	27194..27612	-	419	Protein_25	EamA family transporter	-
HV047_RS00135	27675..28121	-	447	WP_023298965.1	GNAT family N-acetyltransferase	-
HV047_RS00140	28186..28275	-	90	WP_022646551.1	type I toxin-antitoxin system toxin TisB	-
HV047_RS00145	28762..29076	+	315	WP_022646552.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV047_RS00150	29073..29492	+	420	WP_023305145.1	helix-turn-helix domain-containing protein	Antitoxin
HV047_RS00155	29639..29737	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
HV047_RS00160	29844..31532	+	1689	WP_048966211.1	acetolactate synthase large subunit	-
HV047_RS00165	31536..31823	+	288	WP_045336030.1	acetolactate synthase small subunit	-
HV047_RS00170	31949..32542	+	594	WP_022646555.1	transcriptional regulator UhpA	-
HV047_RS00175	32539..34044	+	1506	WP_048966212.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.55 Da
• Isoelectric Point: 10.1989

>T166017 WP_022646552.1 NZ_CP058172:28762-29076 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQYREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T166017 NZ_CP074019:c4289532-4289257 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15508.15 Da
• Isoelectric Point: 5.6761

>AT166017 WP_023305145.1 NZ_CP058172:29073-29492 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAVRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT166017 NZ_CP074019:c4289260-4288901 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATGTATAA

Structures

Toxin

• AlphaFold DB: A0A155SSD8