Detailed information of TA system TA166014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 54351..55078
• Replicon: plasmid pRHBSTW-00059_2
• Accession: NZ_CP058168
• Organism: Enterobacter hormaechei strain RHBSTW-00059

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HV048_RS22865
• Protein ID: WP_011251285.1
• Coordinates: 54767..55078 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV048_RS22860
• Protein ID: WP_011251286.1
• Coordinates: 54351..54770 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV048_RS22830	49729..50736	-	1008	WP_032413314.1	DUF1611 domain-containing protein	-
HV048_RS22835	51221..52429	+	1209	WP_063216414.1	IS256 family transposase	-
HV048_RS22840	52539..53009	-	471	WP_004883378.1	RES family NAD+ phosphorylase	-
HV048_RS22845	53006..53449	-	444	WP_032413443.1	DUF2384 domain-containing protein	-
HV048_RS22850	53550..53779	-	230	Protein_54	DUF4113 domain-containing protein	-
HV048_RS22855	53820..54137	+	318	Protein_55	transposase	-
HV048_RS22860	54351..54770	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HV048_RS22865	54767..55078	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV048_RS22870	55283..56343	-	1061	Protein_58	IS3 family transposase	-
HV048_RS22875	56393..56809	-	417	WP_057072957.1	type II toxin-antitoxin system VapC family toxin	-
HV048_RS22880	56806..57036	-	231	WP_057072956.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HV048_RS22885	57636..57854	+	219	WP_016240352.1	type II toxin-antitoxin system antitoxin CcdA	-
HV048_RS22890	57856..58161	+	306	WP_045616569.1	type II toxin-antitoxin system toxin CcdB	-
HV048_RS22895	58331..59347	+	1017	WP_063216424.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..77568	77568
	-	inside	IScluster/Tn	-	-	51221..63946	12725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T166014 WP_011251285.1 NZ_CP058168:c55078-54767 [Enterobacter hormaechei]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T166014 NZ_CP074019:c3545125-3544790 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT166014 WP_011251286.1 NZ_CP058168:c54770-54351 [Enterobacter hormaechei]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT166014 NZ_CP074019:c3545373-3545125 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1