Detailed information of TA system TA165999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 30479..31209
• Replicon: chromosome
• Accession: NZ_CP058167
• Organism: Enterobacter hormaechei strain RHBSTW-00059

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: HV048_RS00155
• Protein ID: WP_023302825.1
• Coordinates: 30479..30793 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV048_RS00160
• Protein ID: WP_023305145.1
• Coordinates: 30790..31209 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV048_RS00130	26145..27230	+	1086	Protein_25	cellulase family glycosylhydrolase	-
HV048_RS00135	27136..28320	-	1185	WP_023305143.1	multidrug efflux MFS transporter EmrD	-
HV048_RS00140	28496..29329	-	834	WP_023305144.1	DMT family transporter	-
HV048_RS00145	29392..29838	-	447	WP_023298965.1	GNAT family N-acetyltransferase	-
HV048_RS00150	29903..29992	-	90	WP_022646551.1	type I toxin-antitoxin system toxin TisB	-
HV048_RS00155	30479..30793	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV048_RS00160	30790..31209	+	420	WP_023305145.1	helix-turn-helix domain-containing protein	Antitoxin
HV048_RS00165	31356..31454	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
HV048_RS00170	31561..33249	+	1689	WP_032676730.1	acetolactate synthase large subunit	-
HV048_RS00175	33253..33540	+	288	WP_010426528.1	acetolactate synthase small subunit	-
HV048_RS00180	33666..34259	+	594	WP_022646555.1	transcriptional regulator UhpA	-
HV048_RS00185	34256..35761	+	1506	WP_023305147.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T165999 WP_023302825.1 NZ_CP058167:30479-30793 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T165999 NZ_CP074019:278965-279138 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15508.15 Da
• Isoelectric Point: 5.6761

>AT165999 WP_023305145.1 NZ_CP058167:30790-31209 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAVRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT165999 NZ_CP074019:278641-278937 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A837FIL2