Detailed information of TA system TA165960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 97282..97928
• Replicon: chromosome
• Accession: NZ_CP058160
• Organism: Enterobacter hormaechei strain RHBSTW-00083

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV059_RS00495
• Protein ID: WP_047050720.1
• Coordinates: 97578..97928 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7H8UAV4
• Locus tag: HV059_RS00490
• Protein ID: WP_045335986.1
• Coordinates: 97282..97581 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV059_RS00465	92750..93334	-	585	WP_023299002.1	type 1 fimbrial protein	-
HV059_RS00470	93387..93989	-	603	WP_047050717.1	response regulator transcription factor	-
HV059_RS00475	94824..95612	+	789	WP_045329485.1	winged helix-turn-helix domain-containing protein	-
HV059_RS00480	95614..96093	+	480	WP_047050718.1	hypothetical protein	-
HV059_RS00485	96336..97217	+	882	WP_047050719.1	helix-turn-helix domain-containing protein	-
HV059_RS00490	97282..97581	-	300	WP_045335986.1	XRE family transcriptional regulator	Antitoxin
HV059_RS00495	97578..97928	-	351	WP_047050720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV059_RS00500	98201..98453	-	253	Protein_99	DNA-binding protein	-
HV059_RS00505	98556..98897	-	342	WP_006812446.1	SymE family type I addiction module toxin	-
HV059_RS00510	99034..99210	+	177	WP_071962261.1	toxin-antitoxin system HicB family antitoxin	-
HV059_RS00515	99250..100806	-	1557	WP_047050722.1	hypothetical protein	-
HV059_RS00520	101101..101421	-	321	WP_023315132.1	TA system toxin CbtA family protein	-
HV059_RS00525	101455..101820	-	366	WP_045307488.1	type IV toxin-antitoxin system YeeU family antitoxin	-
HV059_RS00530	101852..102073	-	222	WP_023315134.1	DUF987 domain-containing protein	-
HV059_RS00535	102082..102552	-	471	WP_023315135.1	DNA repair protein RadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	94824..118360	23536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13497.59 Da
• Isoelectric Point: 8.8416

>T165960 WP_047050720.1 NZ_CP058160:c97928-97578 [Enterobacter hormaechei]
MWTVLFSKVFEQWLLEQEDGLQEKVLADLLNLQNYGPRLPRPYADTVKGSQYKNMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKRNDKTFYEKLIRIADAEFTLHLTSLEAAK

Nucleotide

• Length: 351 bp

>T165960 NZ_CP074007:c1449684-1449307 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11129.87 Da
• Isoelectric Point: 6.4714

>AT165960 WP_045335986.1 NZ_CP058160:c97581-97282 [Enterobacter hormaechei]
VKTLNEVIASRSPESQQRIQEMAEEMFLETGLQLLREELQLSQKHVADLMGVSQPAVTQLEQRGNDLKIATLKRYIEAMG
GKLSINVELPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT165960 NZ_CP074007:c1450142-1449774 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATTCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7H8UAV4