Detailed information of TA system TA165943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 94677..95323
• Replicon: chromosome
• Accession: NZ_CP058157
• Organism: Enterobacter hormaechei strain RHBSTW-00086

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV061_RS00475
• Protein ID: WP_047050720.1
• Coordinates: 94973..95323 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A9E7BLR3
• Locus tag: HV061_RS00470
• Protein ID: WP_023299004.1
• Coordinates: 94677..94976 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV061_RS00445	90145..90729	-	585	WP_022646603.1	type 1 fimbrial protein	-
HV061_RS00450	90782..91384	-	603	WP_022646604.1	LuxR family transcriptional regulator	-
HV061_RS00455	92219..93007	+	789	WP_063200584.1	winged helix-turn-helix domain-containing protein	-
HV061_RS00460	93009..93488	+	480	WP_017693981.1	hypothetical protein	-
HV061_RS00465	93731..94612	+	882	WP_038415011.1	helix-turn-helix domain-containing protein	-
HV061_RS00470	94677..94976	-	300	WP_023299004.1	XRE family transcriptional regulator	Antitoxin
HV061_RS00475	94973..95323	-	351	WP_047050720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV061_RS00480	95478..95893	-	416	Protein_95	hydroxyacid dehydrogenase	-
HV061_RS00485	95944..96285	-	342	WP_063200578.1	SymE family type I addiction module toxin	-
HV061_RS00490	96411..96602	+	192	WP_038416963.1	toxin-antitoxin system HicB family antitoxin	-
HV061_RS00495	96608..96835	-	228	WP_139153348.1	hypothetical protein	-
HV061_RS00500	96881..97234	-	354	WP_063200576.1	hypothetical protein	-
HV061_RS00505	97445..97765	-	321	WP_071605960.1	TA system toxin CbtA family protein	-
HV061_RS00510	97807..98163	-	357	WP_142667717.1	type IV toxin-antitoxin system YeeU family antitoxin	-
HV061_RS00515	98195..98416	-	222	WP_023315134.1	DUF987 domain-containing protein	-
HV061_RS00520	98425..98895	-	471	WP_032294514.1	DNA repair protein RadC	-
HV061_RS00525	98965..99786	-	822	WP_126493465.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13497.59 Da
• Isoelectric Point: 8.8416

>T165943 WP_047050720.1 NZ_CP058157:c95323-94973 [Enterobacter hormaechei]
MWTVLFSKVFEQWLLEQEDGLQEKVLADLLNLQNYGPRLPRPYADTVKGSQYKNMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKRNDKTFYEKLIRIADAEFTLHLTSLEAAK

Nucleotide

• Length: 351 bp

>T165943 NZ_CP074005:901300-901707 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11101.81 Da
• Isoelectric Point: 5.7151

>AT165943 WP_023299004.1 NZ_CP058157:c94976-94677 [Enterobacter hormaechei]
VKTLNEVIASRSPESQQRIQEMAEEMFLETGLQLLREELQLSQKHVADLMGVSQPAVTQLEQRGNDLKIATLKRYIEAMG
GKLSINVELPTGKQVAFHL

Nucleotide

• Length: 300 bp

>AT165943 NZ_CP074005:901053-901319 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA