Detailed information of TA system TA165877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3864867..3865464
• Replicon: chromosome
• Accession: NZ_CP058131
• Organism: Klebsiella quasipneumoniae strain RHBSTW-00138

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3T0QUS6
• Locus tag: HV092_RS19100
• Protein ID: WP_017899012.1
• Coordinates: 3865147..3865464 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV092_RS19095
• Protein ID: WP_106939351.1
• Coordinates: 3864867..3865154 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV092_RS19065	3860954..3861202	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
HV092_RS19070	3861220..3861561	-	342	WP_004202492.1	RamA family antibiotic efflux transcriptional regulator	-
HV092_RS19075	3861592..3862707	-	1116	WP_181351711.1	MBL fold metallo-hydrolase	-
HV092_RS19080	3862887..3863468	+	582	WP_017899014.1	TetR/AcrR family transcriptional regulator	-
HV092_RS19085	3863468..3863836	+	369	WP_004202487.1	MmcQ/YjbR family DNA-binding protein	-
HV092_RS19090	3863956..3864609	+	654	WP_004202485.1	oxygen-insensitive NAD(P)H nitroreductase	-
HV092_RS19095	3864867..3865154	-	288	WP_106939351.1	helix-turn-helix transcriptional regulator	Antitoxin
HV092_RS19100	3865147..3865464	-	318	WP_017899012.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV092_RS19105	3865648..3866691	-	1044	WP_181351712.1	DUF2157 domain-containing protein	-
HV092_RS19110	3867223..3868092	-	870	WP_181351713.1	helix-turn-helix transcriptional regulator	-
HV092_RS19115	3868201..3869628	+	1428	WP_004202473.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-87)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12185.52 Da
• Isoelectric Point: 11.2767

>T165877 WP_017899012.1 NZ_CP058131:c3865464-3865147 [Klebsiella quasipneumoniae]
MFRMVVHVDVKKELQALPAIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQNKTLFLLRVF
VKKTQKTPLSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T165877 NZ_CP073994:889259-889666 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10868.57 Da
• Isoelectric Point: 10.2213

>AT165877 WP_106939351.1 NZ_CP058131:c3865154-3864867 [Klebsiella quasipneumoniae]
MSKKIIDWDTLKTELLSQPEVQVAFDAQERQHRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRTSLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT165877 NZ_CP073994:889012-889278 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A3T0QUS6