Detailed information of TA system TA165812

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69536..69800
• Replicon: plasmid pRHB02-C22_2
• Accession: NZ_CP058058
• Organism: Escherichia fergusonii strain RHB02-C22

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HVV54_RS23120
• Protein ID: WP_001331364.1
• Coordinates: 69648..69800 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	69536..69593 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVV54_RS23105	64775..67066	-	2292	WP_001289276.1	hypothetical protein	-
HVV54_RS23110	67059..68129	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
HVV54_RS23115	68148..69356	-	1209	WP_181465534.1	IncI1-type conjugal transfer protein TrbA	-
-	69536..69593	-	58	NuclAT_0	-	Antitoxin
-	69536..69593	-	58	NuclAT_0	-	Antitoxin
-	69536..69593	-	58	NuclAT_0	-	Antitoxin
-	69536..69593	-	58	NuclAT_0	-	Antitoxin
HVV54_RS23120	69648..69800	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
HVV54_RS23125	69872..70123	-	252	WP_001291965.1	hypothetical protein	-
HVV54_RS23130	70423..70719	+	297	WP_001275298.1	hypothetical protein	-
HVV54_RS23135	70784..70960	-	177	WP_001054904.1	hypothetical protein	-
HVV54_RS23140	71352..71561	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
HVV54_RS23145	71633..72295	-	663	WP_000653334.1	plasmid IncI1-type surface exclusion protein ExcA	-
HVV54_RS23150	72360..74522	-	2163	WP_000698351.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aadA2 / cmlA1 / ant(3'')-Ia / sul3	-	1..116881	116881

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T165812 WP_001331364.1 NZ_CP058058:69648-69800 [Escherichia fergusonii]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T165812 NZ_CP073981:631320-631598 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT165812 NZ_CP058058:c69593-69536 [Escherichia fergusonii]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin