Detailed information of TA system TA165792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1104749..1105428
• Replicon: chromosome
• Accession: NZ_CP058057
• Organism: Escherichia fergusonii strain RHB02-C22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: HVV54_RS05440
• Protein ID: WP_002431667.1
• Coordinates: 1104749..1105051 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVV54_RS05445
• Protein ID: WP_000806441.1
• Coordinates: 1105087..1105428 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVV54_RS05415	1100125..1101777	+	1653	WP_181203208.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HVV54_RS05420	1101815..1102318	-	504	WP_000667001.1	hypothetical protein	-
HVV54_RS05425	1102315..1103115	-	801	WP_000439795.1	hypothetical protein	-
HVV54_RS05430	1103139..1103618	-	480	WP_181203209.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HVV54_RS05435	1103822..1104616	-	795	WP_000365145.1	TraB/GumN family protein	-
HVV54_RS05440	1104749..1105051	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVV54_RS05445	1105087..1105428	+	342	WP_000806441.1	HigA family addiction module antidote protein	Antitoxin
HVV54_RS05450	1105542..1108046	-	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
HVV54_RS05455	1108353..1109642	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T165792 WP_002431667.1 NZ_CP058057:1104749-1105051 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T165792 NZ_CP073964:c66254-65874 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT165792 WP_000806441.1 NZ_CP058057:1105087-1105428 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT165792 NZ_CP073964:c66475-66254 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8