Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 32484..33345 Replicon plasmid pRHB10-C04_2
Accession NZ_CP057919
Organism Escherichia fergusonii strain RHB10-C04

Toxin (Protein)


Gene name yhaV Uniprot ID A0A7W3G1F8
Locus tag HVW38_RS22205 Protein ID WP_024195926.1
Coordinates 32851..33345 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A7W3G1H0
Locus tag HVW38_RS22200 Protein ID WP_001559862.1
Coordinates 32484..32828 (+) Length 115 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVW38_RS22165 27972..28553 - 582 WP_097700241.1 hypothetical protein -
HVW38_RS22170 28800..29300 + 501 WP_000528932.1 hypothetical protein -
HVW38_RS22175 29313..29594 + 282 WP_181576149.1 hypothetical protein -
HVW38_RS22180 29610..30086 + 477 WP_181576150.1 hypothetical protein -
HVW38_RS22185 30253..31386 + 1134 WP_181576151.1 DUF3800 domain-containing protein -
HVW38_RS22190 31420..31932 - 513 WP_000151784.1 hypothetical protein -
HVW38_RS22195 32163..32240 + 78 Protein_38 helix-turn-helix domain-containing protein -
HVW38_RS22200 32484..32828 + 345 WP_001559862.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
HVW38_RS22205 32851..33345 + 495 WP_024195926.1 type II toxin-antitoxin system YhaV family toxin Toxin
HVW38_RS22210 33527..37333 + 3807 WP_181576152.1 hypothetical protein -
HVW38_RS22215 37864..38214 + 351 WP_000493380.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..114980 114980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(11-147)

Antitoxin

PrlF antitoxin

(16-114)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 19275.36 Da        Isoelectric Point: 8.7864

>T165781 WP_024195926.1 NZ_CP057919:32851-33345 [Escherichia fergusonii]
MEYLEINGWKICFHECFLRQITGLMKKVAELKASKPLEYHKKKETKLLVAIERVIEERITANPLDPQFRQGNTLGEEYKH
WFRAKFLMQFRLFYRCSQEHKTIVIGWVNGFDTLRAYDSRTDAYKVFGEMLKAGNPPDDWDTLLCEAGKATATTNMVDFL
TKRI

Download         Length: 495 bp

>T165781 NZ_CP073962:c2896082-2895699 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 115 a.a.        Molecular weight: 12755.59 Da        Isoelectric Point: 4.6652

>AT165781 WP_001559862.1 NZ_CP057919:32484-32828 [Escherichia fergusonii]
MATQILNSAEVSLRAESRLTERSQTTIPAVIRDALHLKPGEYIHYTLLAGGKVIMSRQNEEQDDPVISQFLTFLENDMKK
NPQKIAPVPTMFWESMKALTAGINVDLDAPLTED

Download         Length: 345 bp

>AT165781 NZ_CP073962:c2896543-2896163 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3G1F8

Antitoxin

Source ID Structure
AlphaFold DB A0A7W3G1H0

References