Detailed information of TA system TA165774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2544227..2544887
• Replicon: chromosome
• Accession: NZ_CP057918
• Organism: Escherichia fergusonii strain RHB10-C04

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HVW38_RS12225
• Protein ID: WP_046077353.1
• Coordinates: 2544534..2544887 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVW38_RS12220
• Protein ID: WP_001553123.1
• Coordinates: 2544227..2544529 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVW38_RS12200	2539586..2540383	+	798	WP_046077351.1	DgsA anti-repressor MtfA	-
HVW38_RS12210	2540721..2541982	+	1262	Protein_2380	integrase arm-type DNA-binding domain-containing protein	-
HVW38_RS12215	2542839..2543723	-	885	WP_046077352.1	integrase domain-containing protein	-
HVW38_RS12220	2544227..2544529	-	303	WP_001553123.1	XRE family transcriptional regulator	Antitoxin
HVW38_RS12225	2544534..2544887	-	354	WP_046077353.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVW38_RS12230	2545384..2545605	-	222	WP_046077354.1	helix-turn-helix domain-containing protein	-
HVW38_RS12235	2545602..2546069	-	468	WP_046077355.1	hypothetical protein	-
HVW38_RS12240	2546956..2547735	+	780	WP_046077393.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13486.42 Da
• Isoelectric Point: 8.9507

>T165774 WP_046077353.1 NZ_CP057918:c2544887-2544534 [Escherichia fergusonii]
VWTIKTTDMFDHWFISLNDTDRASVLAALLVLREKGPGLSRPYADTIRGSRYSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMLPIADREFTNWLNTLKEKE

Nucleotide

• Length: 354 bp

>T165774 NZ_CP073962:1891534-1891788 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11084.82 Da
• Isoelectric Point: 5.1786

>AT165774 WP_001553123.1 NZ_CP057918:c2544529-2544227 [Escherichia fergusonii]
MGRTLEQLIADEKPEVVDEAQAMATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT165774 NZ_CP073962:1891286-1891537 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA