Detailed information of TA system TA165759

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 71697..71961
• Replicon: plasmid pRHB10-C23_2
• Accession: NZ_CP057897
• Organism: Escherichia fergusonii strain RHB10-C23

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: HVW49_RS22420
• Protein ID: WP_024179514.1
• Coordinates: 71809..71961 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	71697..71759 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVW49_RS22405	66936..69227	-	2292	WP_001289276.1	hypothetical protein	-
HVW49_RS22410	69220..70290	-	1071	WP_001542508.1	IncI1-type conjugal transfer protein TrbB	-
HVW49_RS22415	70309..71517	-	1209	WP_175237471.1	IncI1-type conjugal transfer protein TrbA	-
-	71697..71759	-	63	NuclAT_0	-	Antitoxin
-	71697..71759	-	63	NuclAT_0	-	Antitoxin
-	71697..71759	-	63	NuclAT_0	-	Antitoxin
-	71697..71759	-	63	NuclAT_0	-	Antitoxin
HVW49_RS22420	71809..71961	+	153	WP_024179514.1	Hok/Gef family protein	Toxin
HVW49_RS22425	72033..72284	-	252	WP_001291965.1	hypothetical protein	-
-	72678..72729	-	52	NuclAT_1	-	-
-	72678..72729	-	52	NuclAT_1	-	-
-	72678..72729	-	52	NuclAT_1	-	-
-	72678..72729	-	52	NuclAT_1	-	-
HVW49_RS22430	73954..74163	-	210	WP_015059315.1	hemolysin expression modulator Hha	-
HVW49_RS22435	74261..74875	-	615	WP_015059316.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..114980	114980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5795.12 Da
• Isoelectric Point: 8.7948

>T165759 WP_024179514.1 NZ_CP057897:71809-71961 [Escherichia fergusonii]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T165759 NZ_CP073958:c2871109-2870732 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT165759 NZ_CP057897:c71759-71697 [Escherichia fergusonii]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin