Detailed information of TA system TA165751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2544230..2544890
• Replicon: chromosome
• Accession: NZ_CP057896
• Organism: Escherichia fergusonii strain RHB10-C23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HVW49_RS12225
• Protein ID: WP_046077353.1
• Coordinates: 2544537..2544890 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVW49_RS12220
• Protein ID: WP_001553123.1
• Coordinates: 2544230..2544532 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVW49_RS12200	2539589..2540386	+	798	WP_046077351.1	DgsA anti-repressor MtfA	-
HVW49_RS12210	2540724..2541985	+	1262	Protein_2380	integrase arm-type DNA-binding domain-containing protein	-
HVW49_RS12215	2542842..2543726	-	885	WP_046077352.1	integrase domain-containing protein	-
HVW49_RS12220	2544230..2544532	-	303	WP_001553123.1	XRE family transcriptional regulator	Antitoxin
HVW49_RS12225	2544537..2544890	-	354	WP_046077353.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVW49_RS12230	2545387..2545608	-	222	WP_046077354.1	helix-turn-helix domain-containing protein	-
HVW49_RS12235	2545605..2546072	-	468	WP_046077355.1	hypothetical protein	-
HVW49_RS12240	2546959..2547738	+	780	WP_046077393.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13486.42 Da
• Isoelectric Point: 8.9507

>T165751 WP_046077353.1 NZ_CP057896:c2544890-2544537 [Escherichia fergusonii]
VWTIKTTDMFDHWFISLNDTDRASVLAALLVLREKGPGLSRPYADTIRGSRYSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMLPIADREFTNWLNTLKEKE

Nucleotide

• Length: 354 bp

>T165751 NZ_CP073958:1857070-1857324 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11084.82 Da
• Isoelectric Point: 5.1786

>AT165751 WP_001553123.1 NZ_CP057896:c2544532-2544230 [Escherichia fergusonii]
MGRTLEQLIADEKPEVVDEAQAMATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT165751 NZ_CP073958:1856822-1857073 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA