Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 334584..335250 Replicon chromosome
Accession NZ_CP057864
Organism Citrobacter freundii strain RHB12-C19

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag HVW68_RS01550 Protein ID WP_003847996.1
Coordinates 334933..335250 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag HVW68_RS01545 Protein ID WP_003837894.1
Coordinates 334584..334880 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVW68_RS01530 331760..332233 - 474 WP_003023524.1 transcription elongation factor GreB -
HVW68_RS01535 332459..333178 + 720 WP_001157751.1 two-component system response regulator OmpR -
HVW68_RS01540 333175..334527 + 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
HVW68_RS01545 334584..334880 - 297 WP_003837894.1 helix-turn-helix domain-containing protein Antitoxin
HVW68_RS01550 334933..335250 - 318 WP_003847996.1 hypothetical protein Toxin
HVW68_RS01555 335373..336995 - 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
HVW68_RS01560 337374..339092 + 1719 WP_148374155.1 DUF4153 domain-containing protein -
HVW68_RS01565 339202..340080 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T165723 WP_003847996.1 NZ_CP057864:c335250-334933 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T165723 NZ_CP073953:1312357-1312755 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT165723 WP_003837894.1 NZ_CP057864:c334880-334584 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT165723 NZ_CP073953:1312130-1312357 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References