Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 44529..45177 Replicon chromosome
Accession NZ_CP057864
Organism Citrobacter freundii strain RHB12-C19

Toxin (Protein)


Gene name higB Uniprot ID A0A0D7M266
Locus tag HVW68_RS00205 Protein ID WP_016151211.1
Coordinates 44529..44891 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0D7LEH2
Locus tag HVW68_RS00210 Protein ID WP_003840660.1
Coordinates 44878..45177 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVW68_RS00190 40988..42316 + 1329 WP_057101854.1 MFS transporter -
HVW68_RS00195 42459..43850 + 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
HVW68_RS00200 43976..44428 + 453 WP_003023933.1 DUF1198 family protein -
HVW68_RS00205 44529..44891 + 363 WP_016151211.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HVW68_RS00210 44878..45177 + 300 WP_003840660.1 helix-turn-helix transcriptional regulator Antitoxin
HVW68_RS00215 45296..46489 + 1194 WP_003840662.1 purine ribonucleoside efflux pump NepI -
HVW68_RS00220 46538..47920 - 1383 WP_057063284.1 glycoside hydrolase family 1 protein -
HVW68_RS00225 48015..48308 - 294 WP_008323809.1 hypothetical protein -
HVW68_RS00230 48439..48855 + 417 WP_181825881.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13788.66 Da        Isoelectric Point: 5.1976

>T165721 WP_016151211.1 NZ_CP057864:44529-44891 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Download         Length: 363 bp

>T165721 NZ_CP073953:864634-865041 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11016.85 Da        Isoelectric Point: 8.5047

>AT165721 WP_003840660.1 NZ_CP057864:44878-45177 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Download         Length: 300 bp

>AT165721 NZ_CP073953:864387-864653 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7M266

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LEH2

References