Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3710125..3710722 Replicon chromosome
Accession NZ_CP057722
Organism Klebsiella pneumoniae strain RHB16-C24

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HVX23_RS18065 Protein ID WP_004142563.1
Coordinates 3710405..3710722 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HVX23_RS18060 Protein ID WP_004142561.1
Coordinates 3710125..3710412 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX23_RS18030 3706205..3706453 + 249 WP_002893037.1 DUF1158 domain-containing protein -
HVX23_RS18035 3706471..3706812 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HVX23_RS18040 3706843..3707958 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
HVX23_RS18045 3708138..3708719 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
HVX23_RS18050 3708719..3709087 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
HVX23_RS18055 3709207..3709860 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
HVX23_RS18060 3710125..3710412 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HVX23_RS18065 3710405..3710722 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HVX23_RS18070 3710907..3711950 - 1044 WP_019704892.1 DUF2157 domain-containing protein -
HVX23_RS18075 3712620..3713486 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
HVX23_RS18080 3713595..3715022 + 1428 WP_032411778.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T165713 WP_004142563.1 NZ_CP057722:c3710722-3710405 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T165713 NZ_CP073949:c4197780-4197607 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT165713 WP_004142561.1 NZ_CP057722:c3710412-3710125 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT165713 NZ_CP073949:c4198104-4197808 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References