Detailed information of TA system TA165670

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73903..74167
• Replicon: plasmid pRHB18-C04_2
• Accession: NZ_CP057682
• Organism: Escherichia fergusonii strain RHB18-C04

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: HVX35_RS22525
• Protein ID: WP_001303307.1
• Coordinates: 74015..74167 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	73903..73965 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX35_RS22510	69142..71433	-	2292	WP_112868651.1	conjugal transfer protein TrbC	-
HVX35_RS22515	71426..72496	-	1071	WP_181464523.1	IncI1-type conjugal transfer protein TrbB	-
HVX35_RS22520	72515..73723	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	73903..73965	-	63	NuclAT_0	-	Antitoxin
-	73903..73965	-	63	NuclAT_0	-	Antitoxin
-	73903..73965	-	63	NuclAT_0	-	Antitoxin
-	73903..73965	-	63	NuclAT_0	-	Antitoxin
HVX35_RS22525	74015..74167	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
HVX35_RS22530	74239..74490	-	252	WP_001291965.1	hypothetical protein	-
-	74877..74928	-	52	NuclAT_1	-	-
-	74877..74928	-	52	NuclAT_1	-	-
-	74877..74928	-	52	NuclAT_1	-	-
-	74877..74928	-	52	NuclAT_1	-	-
HVX35_RS22535	75414..75590	-	177	WP_001054900.1	hypothetical protein	-
HVX35_RS22540	75982..76191	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
HVX35_RS22545	76263..76925	-	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
HVX35_RS22550	76996..79164	-	2169	WP_000698368.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..117379	117379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T165670 WP_001303307.1 NZ_CP057682:74015-74167 [Escherichia fergusonii]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T165670 NZ_CP073943:3372020-3372238 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT165670 NZ_CP057682:c73965-73903 [Escherichia fergusonii]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin