Detailed information of TA system TA165654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1740608..1741302
• Replicon: chromosome
• Accession: NZ_CP057681
• Organism: Escherichia fergusonii strain RHB18-C04

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: HVX35_RS08575
• Protein ID: WP_181199277.1
• Coordinates: 1740608..1741006 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: HVX35_RS08580
• Protein ID: WP_181199278.1
• Coordinates: 1741009..1741302 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX35_RS08545	1735736..1737193	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
HVX35_RS08550	1737202..1737483	+	282	WP_000106440.1	hypothetical protein	-
HVX35_RS08555	1737492..1738016	-	525	WP_141095971.1	metal-dependent hydrolase	-
HVX35_RS08560	1738078..1738692	-	615	Protein_1677	peptide chain release factor H	-
HVX35_RS08565	1738689..1739828	-	1140	WP_181199275.1	RNA ligase RtcB family protein	-
HVX35_RS08570	1740146..1740598	-	453	WP_181199276.1	GNAT family N-acetyltransferase	-
HVX35_RS08575	1740608..1741006	-	399	WP_181199277.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HVX35_RS08580	1741009..1741302	-	294	WP_181199278.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HVX35_RS08585	1741354..1742409	-	1056	WP_181199279.1	DNA polymerase IV	-
HVX35_RS08590	1742480..1743266	-	787	Protein_1683	putative lateral flagellar export/assembly protein LafU	-
HVX35_RS08595	1743211..1744950	+	1740	WP_181199280.1	flagellar biosynthesis protein FlhA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1667212..1753422	86210

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15530.91 Da
• Isoelectric Point: 8.0934

>T165654 WP_181199277.1 NZ_CP057681:c1741006-1740608 [Escherichia fergusonii]
MRVFKTKLIRLQLTDKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T165654 NZ_CP073943:179732-179839 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTTGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11165.69 Da
• Isoelectric Point: 5.8944

>AT165654 WP_181199278.1 NZ_CP057681:c1741302-1741009 [Escherichia fergusonii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKSIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNGFKE

Nucleotide

• Length: 294 bp

>AT165654 NZ_CP073943:c179684-179618 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT