Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1015876..1016378 Replicon chromosome
Accession NZ_CP057681
Organism Escherichia fergusonii strain RHB18-C04

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HVX35_RS05030 Protein ID WP_000767829.1
Coordinates 1015876..1016130 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HVX35_RS05035 Protein ID WP_001259255.1
Coordinates 1016127..1016378 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX35_RS05000 1010892..1011119 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
HVX35_RS05005 1011133..1012191 - 1059 WP_180491514.1 transport protein YeeE -
HVX35_RS05010 1012370..1013728 - 1359 WP_000019201.1 putrescine/proton symporter PlaP -
HVX35_RS05015 1013718..1013780 - 63 WP_010723108.1 membrane protein YoeI -
HVX35_RS05020 1013994..1014923 - 930 WP_000803361.1 LysR family transcriptional regulator -
HVX35_RS05025 1014969..1015793 - 825 WP_000754726.1 SDR family oxidoreductase -
HVX35_RS05030 1015876..1016130 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HVX35_RS05035 1016127..1016378 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HVX35_RS05040 1016662..1016712 + 51 WP_001364200.1 his operon leader peptide -
HVX35_RS05045 1016858..1017757 + 900 WP_000131779.1 ATP phosphoribosyltransferase -
HVX35_RS05050 1017763..1019067 + 1305 WP_015953522.1 histidinol dehydrogenase -
HVX35_RS05055 1019064..1020134 + 1071 WP_181464465.1 histidinol-phosphate transaminase -
HVX35_RS05060 1020134..1021201 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T165652 WP_000767829.1 NZ_CP057681:c1016130-1015876 [Escherichia fergusonii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T165652 NZ_CP073939:c4391437-4391126 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT165652 WP_001259255.1 NZ_CP057681:c1016378-1016127 [Escherichia fergusonii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT165652 NZ_CP073939:c4391125-4390835 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAAGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTACTG
CGAGCGATTGCCAATGATCCGCAACATGTACTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References