Detailed information of TA system TA165639

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40263..40527
• Replicon: plasmid pRHB19-C05_3
• Accession: NZ_CP057659
• Organism: Escherichia fergusonii strain RHB19-C05

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HVX45_RS22975
• Protein ID: WP_001331364.1
• Coordinates: 40375..40527 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	40263..40325 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX45_RS22960	35502..37793	-	2292	WP_109444318.1	conjugal transfer protein TrbC	-
HVX45_RS22965	37786..38856	-	1071	WP_001542508.1	IncI1-type conjugal transfer protein TrbB	-
HVX45_RS22970	38875..40083	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	40263..40325	-	63	NuclAT_0	-	Antitoxin
-	40263..40325	-	63	NuclAT_0	-	Antitoxin
-	40263..40325	-	63	NuclAT_0	-	Antitoxin
-	40263..40325	-	63	NuclAT_0	-	Antitoxin
HVX45_RS22975	40375..40527	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
HVX45_RS22980	40599..40850	-	252	WP_001291964.1	hypothetical protein	-
HVX45_RS22985	41150..41446	+	297	WP_001275298.1	hypothetical protein	-
HVX45_RS22990	41511..41687	-	177	WP_001054904.1	hypothetical protein	-
HVX45_RS22995	42079..42288	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
HVX45_RS23000	42360..43010	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
HVX45_RS23005	43084..45252	-	2169	WP_000698357.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..89313	89313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T165639 WP_001331364.1 NZ_CP057659:40375-40527 [Escherichia fergusonii]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T165639 NZ_CP073939:2300087-2301409 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCTGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGATTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT165639 NZ_CP057659:c40325-40263 [Escherichia fergusonii]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin