Detailed information of TA system TA165638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4097986..4098665
• Replicon: chromosome
• Accession: NZ_CP057657
• Organism: Escherichia fergusonii strain RHB19-C05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: HVX45_RS19990
• Protein ID: WP_002431667.1
• Coordinates: 4097986..4098288 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVX45_RS19995
• Protein ID: WP_000806440.1
• Coordinates: 4098324..4098665 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX45_RS19965	4093362..4095014	+	1653	WP_046082793.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HVX45_RS19970	4095052..4095555	-	504	WP_046082794.1	hypothetical protein	-
HVX45_RS19975	4095552..4096352	-	801	WP_046082795.1	hypothetical protein	-
HVX45_RS19980	4096376..4096855	-	480	WP_000186629.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HVX45_RS19985	4097059..4097853	-	795	WP_046082796.1	TraB/GumN family protein	-
HVX45_RS19990	4097986..4098288	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVX45_RS19995	4098324..4098665	+	342	WP_000806440.1	HigA family addiction module antidote protein	Antitoxin
HVX45_RS20000	4098779..4101283	-	2505	WP_181672380.1	copper-exporting P-type ATPase CopA	-
HVX45_RS20005	4101590..4102879	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T165638 WP_002431667.1 NZ_CP057657:4097986-4098288 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T165638 NZ_CP073939:1948762-1948865 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13157.08 Da
• Isoelectric Point: 5.7737

>AT165638 WP_000806440.1 NZ_CP057657:4098324-4098665 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT165638 NZ_CP073939:c1948903-1948632 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8