Detailed information of TA system TA165637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3905015..3905742
• Replicon: chromosome
• Accession: NZ_CP057657
• Organism: Escherichia fergusonii strain RHB19-C05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0L6ZX69
• Locus tag: HVX45_RS18920
• Protein ID: WP_000547560.1
• Coordinates: 3905015..3905326 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVX45_RS18925
• Protein ID: WP_000126291.1
• Coordinates: 3905323..3905742 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX45_RS18890	3900155..3901864	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
HVX45_RS18895	3901874..3902416	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
HVX45_RS18900	3902416..3903183	+	768	WP_000067390.1	formate hydrogenlyase subunit HycG	-
HVX45_RS18905	3903180..3903590	+	411	WP_001291926.1	formate hydrogenlyase assembly protein HycH	-
HVX45_RS18910	3903583..3904053	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HVX45_RS18915	3904096..3904851	+	756	WP_046083124.1	hypothetical protein	-
HVX45_RS18920	3905015..3905326	+	312	WP_000547560.1	type II toxin-antitoxin system HigB family toxin	Toxin
HVX45_RS18925	3905323..3905742	+	420	WP_000126291.1	helix-turn-helix domain-containing protein	Antitoxin
HVX45_RS18930	3905822..3907246	-	1425	WP_046083147.1	6-phospho-beta-glucosidase AscB	-
HVX45_RS18935	3907255..3908712	-	1458	WP_046083123.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HVX45_RS18940	3908969..3909982	+	1014	WP_072274220.1	DNA-binding transcriptional regulator AscG	-
HVX45_RS18945	3910131..3910658	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12423.12 Da
• Isoelectric Point: 9.7106

>T165637 WP_000547560.1 NZ_CP057657:3905015-3905326 [Escherichia fergusonii]
MHIISKAPFEESARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T165637 NZ_CP073939:975137-975472 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAAGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCTTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACACTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15439.46 Da
• Isoelectric Point: 4.5628

>AT165637 WP_000126291.1 NZ_CP057657:3905323-3905742 [Escherichia fergusonii]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNHEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT165637 NZ_CP073939:974889-975137 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATTCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGGCATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0L6ZX69