Detailed information of TA system TA165598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2459894..2460456
• Replicon: chromosome
• Accession: NZ_CP057632
• Organism: Citrobacter sp. RHB20-C15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6Z3F2
• Locus tag: HVX63_RS11810
• Protein ID: WP_044257939.1
• Coordinates: 2459894..2460172 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVX63_RS11815
• Protein ID: WP_043000693.1
• Coordinates: 2460172..2460456 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX63_RS11790	2455019..2455450	-	432	WP_043000697.1	peroxiredoxin OsmC	-
HVX63_RS11795	2455678..2455821	+	144	WP_042319017.1	stationary-phase-induced ribosome-associated protein	-
HVX63_RS11800	2455993..2457690	+	1698	WP_044257943.1	malate dehydrogenase	-
HVX63_RS11805	2457999..2459714	+	1716	WP_044257940.1	ATP-binding cassette domain-containing protein	-
HVX63_RS11810	2459894..2460172	+	279	WP_044257939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVX63_RS11815	2460172..2460456	+	285	WP_043000693.1	HigA family addiction module antidote protein	Antitoxin
HVX63_RS11820	2460501..2461103	-	603	WP_044257938.1	inorganic diphosphatase	-
HVX63_RS11825	2461230..2461886	-	657	WP_043000691.1	formate dehydrogenase-N subunit gamma	-
HVX63_RS11830	2461879..2462763	-	885	WP_043000690.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10525.03 Da
• Isoelectric Point: 8.1206

>T165598 WP_044257939.1 NZ_CP057632:2459894-2460172 [Citrobacter sp. RHB20-C15]
MIMNFRHKGLRDLFLHGKTSGVLAKHVKRLRHRLAVIDAAGCVNDINMPGYRLHPLSGDRDGVWSISVSGNWRMTFEFVN
GDAYILDDEDYH

Nucleotide

• Length: 279 bp

>T165598 NZ_CP073932:973211-973546 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10370.07 Da
• Isoelectric Point: 10.2982

>AT165598 WP_043000693.1 NZ_CP057632:2460172-2460456 [Citrobacter sp. RHB20-C15]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAQAQ
KSVDVSRLRRLAVQ

Nucleotide

• Length: 285 bp

>AT165598 NZ_CP073932:972963-973211 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7L6Z3F2