Detailed information of TA system TA165579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2458829..2459391
• Replicon: chromosome
• Accession: NZ_CP057630
• Organism: Citrobacter sp. RHB20-C16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6Z3F2
• Locus tag: HVX64_RS11810
• Protein ID: WP_044257939.1
• Coordinates: 2458829..2459107 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVX64_RS11815
• Protein ID: WP_043000693.1
• Coordinates: 2459107..2459391 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX64_RS11790	2453954..2454385	-	432	WP_043000697.1	peroxiredoxin OsmC	-
HVX64_RS11795	2454613..2454756	+	144	WP_042319017.1	stationary-phase-induced ribosome-associated protein	-
HVX64_RS11800	2454928..2456625	+	1698	WP_044257943.1	malate dehydrogenase	-
HVX64_RS11805	2456934..2458649	+	1716	WP_044257940.1	ATP-binding cassette domain-containing protein	-
HVX64_RS11810	2458829..2459107	+	279	WP_044257939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVX64_RS11815	2459107..2459391	+	285	WP_043000693.1	HigA family addiction module antidote protein	Antitoxin
HVX64_RS11820	2459436..2460038	-	603	WP_044257938.1	inorganic diphosphatase	-
HVX64_RS11825	2460165..2460821	-	657	WP_043000691.1	formate dehydrogenase-N subunit gamma	-
HVX64_RS11830	2460814..2461698	-	885	WP_043000690.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10525.03 Da
• Isoelectric Point: 8.1206

>T165579 WP_044257939.1 NZ_CP057630:2458829-2459107 [Citrobacter sp. RHB20-C16]
MIMNFRHKGLRDLFLHGKTSGVLAKHVKRLRHRLAVIDAAGCVNDINMPGYRLHPLSGDRDGVWSISVSGNWRMTFEFVN
GDAYILDDEDYH

Nucleotide

• Length: 279 bp

>T165579 NZ_CP073929:2051375-2051478 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10370.07 Da
• Isoelectric Point: 10.2982

>AT165579 WP_043000693.1 NZ_CP057630:2459107-2459391 [Citrobacter sp. RHB20-C16]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAQAQ
KSVDVSRLRRLAVQ

Nucleotide

• Length: 285 bp

>AT165579 NZ_CP073929:c2051516-2051245 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTAGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A7L6Z3F2