Detailed information of TA system TA165547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1329..1975
• Replicon: plasmid pRHB23-C01_4
• Accession: NZ_CP057568
• Organism: Escherichia fergusonii strain RHB23-C01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3P1YQM6
• Locus tag: HVX91_RS22965
• Protein ID: WP_025667955.1
• Coordinates: 1628..1975 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVX91_RS22960
• Protein ID: WP_001673379.1
• Coordinates: 1329..1628 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX91_RS22945	25..297	-	273	WP_000148348.1	helix-turn-helix domain-containing protein	-
HVX91_RS22950	363..743	-	381	WP_000061763.1	hypothetical protein	-
HVX91_RS22955	1011..1295	+	285	WP_033816863.1	hypothetical protein	-
HVX91_RS22960	1329..1628	-	300	WP_001673379.1	XRE family transcriptional regulator	Antitoxin
HVX91_RS22965	1628..1975	-	348	WP_025667955.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVX91_RS22970	2220..2483	-	264	WP_000424604.1	hypothetical protein	-
HVX91_RS22975	2507..2794	-	288	WP_000356589.1	hypothetical protein	-
HVX91_RS22980	3438..4274	-	837	WP_097294329.1	RepA	-
HVX91_RS22985	4597..5151	-	555	WP_042038368.1	recombinase family protein	-
HVX91_RS22990	5215..6186	+	972	WP_181812623.1	phage tail protein	-
HVX91_RS22995	6189..6722	+	534	WP_181812618.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48686	48686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13530.50 Da
• Isoelectric Point: 9.3634

>T165547 WP_025667955.1 NZ_CP057568:c1975-1628 [Escherichia fergusonii]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKNLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T165547 NZ_CP073924:c4337284-4337111 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11069.90 Da
• Isoelectric Point: 5.8899

>AT165547 WP_001673379.1 NZ_CP057568:c1628-1329 [Escherichia fergusonii]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT165547 NZ_CP073924:c4337608-4337312 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A3P1YQM6