Detailed information of TA system TA165542

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81866..82130
• Replicon: plasmid pRHB23-C01_2
• Accession: NZ_CP057566
• Organism: Escherichia fergusonii strain RHB23-C01

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: HVX91_RS22160
• Protein ID: WP_001303307.1
• Coordinates: 81978..82130 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	81866..81928 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX91_RS22145	77105..79396	-	2292	WP_001289267.1	hypothetical protein	-
HVX91_RS22150	79389..80459	-	1071	WP_047088495.1	IncI1-type conjugal transfer protein TrbB	-
HVX91_RS22155	80478..81686	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	81866..81928	-	63	NuclAT_0	-	Antitoxin
-	81866..81928	-	63	NuclAT_0	-	Antitoxin
-	81866..81928	-	63	NuclAT_0	-	Antitoxin
-	81866..81928	-	63	NuclAT_0	-	Antitoxin
HVX91_RS22160	81978..82130	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
HVX91_RS22165	82202..82453	-	252	WP_001291965.1	hypothetical protein	-
HVX91_RS22170	83112..83288	-	177	WP_001054904.1	hypothetical protein	-
HVX91_RS22175	83497..83706	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
HVX91_RS22180	83804..84418	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
HVX91_RS22185	84494..86662	-	2169	WP_181812593.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..124527	124527

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T165542 WP_001303307.1 NZ_CP057566:81978-82130 [Escherichia fergusonii]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T165542 NZ_CP073924:3501527-3501745 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT165542 NZ_CP057566:c81928-81866 [Escherichia fergusonii]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin