Detailed information of TA system TA165531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3466409..3467103
• Replicon: chromosome
• Accession: NZ_CP057565
• Organism: Escherichia fergusonii strain RHB23-C01

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: HVX91_RS16650
• Protein ID: WP_181811876.1
• Coordinates: 3466409..3466807 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HVX91_RS16655
• Protein ID: WP_000554758.1
• Coordinates: 3466810..3467103 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX91_RS16625	3461773..3462231	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
HVX91_RS16630	3462492..3463949	+	1458	WP_001293012.1	cytosol nonspecific dipeptidase	-
HVX91_RS16635	3464006..3464620	-	615	WP_104917898.1	peptide chain release factor H	-
HVX91_RS16640	3464617..3465756	-	1140	WP_181811875.1	RNA ligase RtcB family protein	-
HVX91_RS16645	3465947..3466399	-	453	WP_088542288.1	GNAT family N-acetyltransferase	-
HVX91_RS16650	3466409..3466807	-	399	WP_181811876.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HVX91_RS16655	3466810..3467103	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HVX91_RS16660	3467155..3468210	-	1056	WP_181811877.1	DNA polymerase IV	-
HVX91_RS16665	3468281..3469066	-	786	WP_181202789.1	putative lateral flagellar export/assembly protein LafU	-
HVX91_RS16670	3469011..3470750	+	1740	WP_181811878.1	flagellar biosynthesis protein FlhA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15503.94 Da
• Isoelectric Point: 7.3840

>T165531 WP_181811876.1 NZ_CP057565:c3466807-3466409 [Escherichia fergusonii]
MRVFKTKLIRLQLTDKELDALTADFISYKRDGVLPDIFGCDALYDDLFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T165531 NZ_CP073924:935527-935934 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT165531 WP_000554758.1 NZ_CP057565:c3467103-3466810 [Escherichia fergusonii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT165531 NZ_CP073924:935280-935546 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: S1QAE3