Detailed information of TA system TA165454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2354455..2355017
• Replicon: chromosome
• Accession: NZ_CP057506
• Organism: Citrobacter freundii strain RHB24-C05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HVY06_RS11370
• Protein ID: WP_096757033.1
• Coordinates: 2354455..2354733 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVY06_RS11375
• Protein ID: WP_096757032.1
• Coordinates: 2354733..2355017 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY06_RS11350	2349564..2349995	-	432	WP_096757037.1	OsmC family protein	-
HVY06_RS11355	2350224..2350364	+	141	WP_096757036.1	stationary-phase-induced ribosome-associated protein	-
HVY06_RS11360	2350544..2352241	+	1698	WP_115258488.1	malate dehydrogenase	-
HVY06_RS11365	2352552..2354267	+	1716	WP_181819369.1	ATP-binding cassette domain-containing protein	-
HVY06_RS11370	2354455..2354733	+	279	WP_096757033.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVY06_RS11375	2354733..2355017	+	285	WP_096757032.1	HigA family addiction module antidote protein	Antitoxin
HVY06_RS11380	2355122..2355778	-	657	WP_096757031.1	formate dehydrogenase-N subunit gamma	-
HVY06_RS11385	2355771..2356655	-	885	WP_115258486.1	formate dehydrogenase N subunit beta	-
HVY06_RS11390	2356669..2359716	-	3048	WP_137398860.1	formate dehydrogenase-N subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10601.05 Da
• Isoelectric Point: 9.5320

>T165454 WP_096757033.1 NZ_CP057506:2354455-2354733 [Citrobacter freundii]
MIMNFRHKGLRDLFLQGRTSGVTAKHVRRLRHRLAVIDAASHVTDINMPGYKLHALTGDRDGVWSISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T165454 NZ_CP073883:395274-395483 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10327.05 Da
• Isoelectric Point: 8.8094

>AT165454 WP_096757032.1 NZ_CP057506:2354733-2355017 [Citrobacter freundii]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNAWSLAEAQ
KSVDISRLRKLVAQ

Nucleotide

• Length: 285 bp

>AT165454 NZ_CP073883:394974-395267 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA