Detailed information of TA system TA165449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 311371..312037
• Replicon: chromosome
• Accession: NZ_CP057506
• Organism: Citrobacter freundii strain RHB24-C05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HVY06_RS01480
• Protein ID: WP_117342464.1
• Coordinates: 311720..312037 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4U6IRD5
• Locus tag: HVY06_RS01475
• Protein ID: WP_003837894.1
• Coordinates: 311371..311667 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY06_RS01460	308547..309020	-	474	WP_003023524.1	transcription elongation factor GreB	-
HVY06_RS01465	309247..309966	+	720	WP_001157751.1	two-component system response regulator OmpR	-
HVY06_RS01470	309963..311315	+	1353	WP_096759268.1	two-component system sensor histidine kinase EnvZ	-
HVY06_RS01475	311371..311667	-	297	WP_003837894.1	helix-turn-helix domain-containing protein	Antitoxin
HVY06_RS01480	311720..312037	-	318	WP_117342464.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVY06_RS01485	312161..313783	-	1623	WP_115257300.1	phosphoenolpyruvate carboxykinase (ATP)	-
HVY06_RS01490	314162..315880	+	1719	WP_181819879.1	DUF4153 domain-containing protein	-
HVY06_RS01495	315931..316809	-	879	WP_096759272.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12226.13 Da
• Isoelectric Point: 9.8310

>T165449 WP_117342464.1 NZ_CP057506:c312037-311720 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSDMLI

Nucleotide

• Length: 318 bp

>T165449 NZ_CP073881:682079-682288 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11157.93 Da
• Isoelectric Point: 7.2050

>AT165449 WP_003837894.1 NZ_CP057506:c311667-311371 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Nucleotide

• Length: 297 bp

>AT165449 NZ_CP073881:681779-682072 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A4U6IRD5