Detailed information of TA system TA165362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 599152..599951
• Replicon: chromosome
• Accession: NZ_CP057481
• Organism: Escherichia fergusonii strain RHB25-C10

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: HVY20_RS02865
• Protein ID: WP_000347270.1
• Coordinates: 599152..599616 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: HVY20_RS02870
• Protein ID: WP_104919918.1
• Coordinates: 599616..599951 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY20_RS02835	594153..594587	-	435	WP_032303830.1	PTS sugar transporter subunit IIA	-
HVY20_RS02840	594605..595483	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HVY20_RS02845	595473..596252	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HVY20_RS02850	596263..596736	-	474	WP_032303829.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HVY20_RS02855	596759..598039	-	1281	WP_181203097.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HVY20_RS02860	598288..599097	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HVY20_RS02865	599152..599616	-	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HVY20_RS02870	599616..599951	-	336	WP_104919918.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HVY20_RS02875	600100..601671	-	1572	WP_181203098.1	galactarate dehydratase	-
HVY20_RS02880	602142..602912	+	771	WP_001058567.1	2-dehydro-3-deoxyglucarate aldolase	-
HVY20_RS02885	602937..603827	+	891	WP_000178100.1	2-hydroxy-3-oxopropionate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T165362 WP_000347270.1 NZ_CP057481:c599616-599152 [Escherichia fergusonii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T165362 NZ_CP073835:2285511-2285699 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12349.93 Da
• Isoelectric Point: 4.5392

>AT165362 WP_104919918.1 NZ_CP057481:c599951-599616 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGRKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT165362 NZ_CP073835:2285229-2285486 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90