Detailed information of TA system TA165357

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51938..52202
• Replicon: plasmid pRHB26-C03_2
• Accession: NZ_CP057464
• Organism: Escherichia fergusonii strain RHB26-C03

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: HVY27_RS22805
• Protein ID: WP_001303307.1
• Coordinates: 52050..52202 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	51938..52000 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY27_RS22790	47177..49468	-	2292	WP_072279833.1	conjugal transfer protein TrbC	-
HVY27_RS22795	49461..50531	-	1071	WP_052895276.1	IncI1-type conjugal transfer protein TrbB	-
HVY27_RS22800	50550..51758	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	51938..52000	-	63	NuclAT_0	-	Antitoxin
-	51938..52000	-	63	NuclAT_0	-	Antitoxin
-	51938..52000	-	63	NuclAT_0	-	Antitoxin
-	51938..52000	-	63	NuclAT_0	-	Antitoxin
HVY27_RS22805	52050..52202	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
HVY27_RS22810	52274..52525	-	252	WP_001291965.1	hypothetical protein	-
HVY27_RS22815	52666..53316	+	651	WP_024244070.1	IS66 family insertion sequence hypothetical protein	-
HVY27_RS22820	53316..53663	+	348	WP_024244069.1	IS66 family insertion sequence element accessory protein TnpB	-
HVY27_RS22825	53683..55254	+	1572	WP_115978212.1	IS66 family transposase	-
HVY27_RS22830	55398..55537	+	140	Protein_63	transposase domain-containing protein	-
HVY27_RS22835	55579..56106	-	528	WP_000182867.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..107745	107745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T165357 WP_001303307.1 NZ_CP057464:52050-52202 [Escherichia fergusonii]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T165357 NZ_CP073830:2248207-2248395 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT165357 NZ_CP057464:c52000-51938 [Escherichia fergusonii]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin