Detailed information of TA system TA165329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 166886..167613
• Replicon: plasmid pRHB26-C08_2
• Accession: NZ_CP057460
• Organism: Klebsiella pneumoniae strain RHB26-C08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HVY29_RS26955
• Protein ID: WP_011251285.1
• Coordinates: 167302..167613 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVY29_RS26950
• Protein ID: WP_011251286.1
• Coordinates: 166886..167305 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY29_RS26910	161939..162961	-	1023	WP_181672731.1	IS21-like element ISSen3 family transposase	-
HVY29_RS26915	163049..163374	+	326	Protein_170	transposase	-
HVY29_RS26920	163970..164149	-	180	WP_007372130.1	hypothetical protein	-
HVY29_RS26925	164228..164925	+	698	Protein_172	IS1 family transposase	-
HVY29_RS26930	165074..165544	-	471	WP_004883378.1	RES family NAD+ phosphorylase	-
HVY29_RS26935	165541..165984	-	444	WP_032413443.1	DUF2384 domain-containing protein	-
HVY29_RS26940	166085..166314	-	230	Protein_175	DUF4113 domain-containing protein	-
HVY29_RS26945	166355..166672	+	318	Protein_176	transposase	-
HVY29_RS26950	166886..167305	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HVY29_RS26955	167302..167613	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HVY29_RS26960	167818..168833	-	1016	Protein_179	IS3 family transposase	-
HVY29_RS26965	168892..170484	-	1593	WP_004902302.1	IS66-like element ISKox1 family transposase	-
HVY29_RS26970	170515..170865	-	351	WP_004189161.1	IS66 family insertion sequence element accessory protein TnpB	-
HVY29_RS26975	170862..171302	-	441	WP_004189163.1	IS66 family insertion sequence hypothetical protein	-
HVY29_RS26980	171388..171875	-	488	Protein_183	transposase	-
HVY29_RS26985	172114..172440	+	327	Protein_184	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..256547	256547
	-	inside	IScluster/Tn	-	-	161160..174439	13279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T165329 WP_011251285.1 NZ_CP057460:c167613-167302 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T165329 NZ_CP073809:c1855039-1854686 [Macrococcus equipercicus]
ATGAAACGCGGGGATGTTTATTTAGCGGATTTATCTCCTGTGAAAGGTTCTGAACAAGGTGGCAAAAGACCGGTTGTCAT
CATTCAGAATGATGTGGGAAATAAGTTCAGTCCGACAGTGATTGTAGCGGCTATAACCGCCAAAATTAATAAAGCGCGCA
TTCCGACACATGTAGAAATCGGACGGACTAATCATCAGCTGGATAAGGATTCAGTGATTCTGCTGGAGCAGATCAGAACG
ATTGATAAAAACAGATTGATAGATAAGCTGACTGTATTATCTCCTGACAAAATGGATGAGGTCGATGAGGCGCTTGAAAT
CAGTCTTGGCCTGAGTGGCCGGTCTCAGGAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT165329 WP_011251286.1 NZ_CP057460:c167305-166886 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT165329 NZ_CP073809:c1855183-1855040 [Macrococcus equipercicus]
ATGAAGAAGTTTGAACAATTACTTGCTGAAGGATATCAATCAATGGCAGAACTGAACTTGATGATTTCTTACGAGCAATT
TGAACTCGAGTGCGAGGCAAGCGAACAGAGTGATTGCTTCTTTGCATCTGAAGATGGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1