Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2614179..2614681 Replicon chromosome
Accession NZ_CP057458
Organism Escherichia fergusonii strain RHB26-C09

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HVY30_RS12555 Protein ID WP_000767829.1
Coordinates 2614179..2614433 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HVY30_RS12560 Protein ID WP_001259255.1
Coordinates 2614430..2614681 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY30_RS12525 2609195..2609422 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
HVY30_RS12530 2609436..2610494 - 1059 WP_104918480.1 transport protein YeeE -
HVY30_RS12535 2610673..2612031 - 1359 WP_000019201.1 putrescine/proton symporter PlaP -
HVY30_RS12540 2612021..2612083 - 63 WP_010723108.1 membrane protein YoeI -
HVY30_RS12545 2612297..2613226 - 930 WP_000803361.1 LysR family transcriptional regulator -
HVY30_RS12550 2613272..2614096 - 825 WP_000754745.1 SDR family oxidoreductase -
HVY30_RS12555 2614179..2614433 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HVY30_RS12560 2614430..2614681 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HVY30_RS12565 2614965..2615015 + 51 WP_001364200.1 his operon leader peptide -
HVY30_RS12570 2615161..2616060 + 900 WP_000131779.1 ATP phosphoribosyltransferase -
HVY30_RS12575 2616066..2617370 + 1305 WP_002431466.1 histidinol dehydrogenase -
HVY30_RS12580 2617367..2618437 + 1071 WP_181669902.1 histidinol-phosphate transaminase -
HVY30_RS12585 2618437..2619504 + 1068 WP_181669903.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T165298 WP_000767829.1 NZ_CP057458:c2614433-2614179 [Escherichia fergusonii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T165298 NZ_CP073791:c4848276-4847929 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT165298 WP_001259255.1 NZ_CP057458:c2614681-2614430 [Escherichia fergusonii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT165298 NZ_CP073791:c4847929-4847630 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References