Detailed information of TA system TA165280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3492196..3492995
• Replicon: chromosome
• Accession: NZ_CP057357
• Organism: Escherichia fergusonii strain RHB28-C21

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: HVY57_RS16845
• Protein ID: WP_000347270.1
• Coordinates: 3492196..3492660 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: HVY57_RS16850
• Protein ID: WP_046082024.1
• Coordinates: 3492660..3492995 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY57_RS16815	3487197..3487631	-	435	WP_000948819.1	PTS sugar transporter subunit IIA	-
HVY57_RS16820	3487649..3488527	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HVY57_RS16825	3488517..3489296	-	780	WP_000406227.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HVY57_RS16830	3489307..3489780	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HVY57_RS16835	3489803..3491083	-	1281	WP_104917761.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HVY57_RS16840	3491332..3492141	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HVY57_RS16845	3492196..3492660	-	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HVY57_RS16850	3492660..3492995	-	336	WP_046082024.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HVY57_RS16855	3493144..3494715	-	1572	WP_104917760.1	galactarate dehydratase	-
HVY57_RS16860	3495186..3495956	+	771	WP_181672904.1	2-dehydro-3-deoxyglucarate aldolase	-
HVY57_RS16865	3495981..3496871	+	891	WP_000178100.1	2-hydroxy-3-oxopropionate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T165280 WP_000347270.1 NZ_CP057357:c3492660-3492196 [Escherichia fergusonii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T165280 NZ_CP057357:c3492660-3492196 [Escherichia fergusonii]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.91 Da
• Isoelectric Point: 4.4128

>AT165280 WP_046082024.1 NZ_CP057357:c3492995-3492660 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDPTMNAFLRFLDADIQNN
PQKTRPFDIQQGRKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT165280 NZ_CP057357:c3492995-3492660 [Escherichia fergusonii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGGCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCCTACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAGGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90