Detailed information of TA system TA165265

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1084982..1085207
• Replicon: chromosome
• Accession: NZ_CP057357
• Organism: Escherichia fergusonii strain RHB28-C21

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: HVY57_RS05400
• Protein ID: WP_000813254.1
• Coordinates: 1084982..1085137 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1085149..1085207 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY57_RS05365	1080215..1080601	-	387	WP_023155117.1	phage holin family protein	-
HVY57_RS05370	1080768..1081628	-	861	WP_048949149.1	hypothetical protein	-
HVY57_RS05375	1081762..1082451	-	690	WP_181199096.1	antiterminator	-
HVY57_RS05380	1082448..1082807	-	360	WP_089585352.1	RusA family crossover junction endodeoxyribonuclease	-
HVY57_RS05385	1082820..1083869	-	1050	WP_048949148.1	DUF968 domain-containing protein	-
HVY57_RS05390	1083871..1084149	-	279	WP_024175747.1	hypothetical protein	-
HVY57_RS05395	1084446..1084838	-	393	WP_000737636.1	hypothetical protein	-
HVY57_RS05400	1084982..1085137	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	1085149..1085207	+	59	-	-	Antitoxin
HVY57_RS05405	1085375..1086040	-	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
HVY57_RS05410	1086215..1086639	-	425	Protein_1070	DUF977 family protein	-
HVY57_RS05415	1086680..1087750	-	1071	WP_181673093.1	phage replisome organizer	-
HVY57_RS05420	1087822..1088247	-	426	WP_000693850.1	Rha family transcriptional regulator	-
HVY57_RS05425	1088244..1088459	-	216	WP_060615121.1	transcriptional regulator	-
HVY57_RS05430	1088509..1089225	+	717	WP_001394126.1	helix-turn-helix domain-containing protein	-
HVY57_RS05435	1089498..1089653	+	156	WP_000379575.1	DUF1391 family protein	-
HVY57_RS05440	1089813..1090040	+	228	WP_181673094.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	hcp2/tssD2	1056574..1110650	54076
	-	flank	IS/Tn	-	-	1090110..1091318	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T165265 WP_000813254.1 NZ_CP057357:c1085137-1084982 [Escherichia fergusonii]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T165265 NZ_CP073783:754406-754816 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATTCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT165265 NZ_CP057357:1085149-1085207 [Escherichia fergusonii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin