Detailed information of TA system TA165202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 349830..350476
• Replicon: chromosome
• Accession: NZ_CP057313
• Organism: Klebsiella pneumoniae strain RHB30-C05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6G4MDN2
• Locus tag: HVY73_RS01615
• Protein ID: WP_064148407.1
• Coordinates: 349830..350156 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVY73_RS01620
• Protein ID: WP_064148406.1
• Coordinates: 350177..350476 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY73_RS01605	345756..347189	+	1434	WP_002920564.1	glycogen synthase GlgA	-
HVY73_RS01610	347207..349654	+	2448	WP_002920561.1	glycogen phosphorylase	-
HVY73_RS01615	349830..350156	+	327	WP_064148407.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVY73_RS01620	350177..350476	+	300	WP_064148406.1	helix-turn-helix transcriptional regulator	Antitoxin
HVY73_RS01625	350539..352047	-	1509	WP_064148405.1	glycerol-3-phosphate dehydrogenase	-
HVY73_RS01630	352252..352581	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
HVY73_RS01635	352632..353462	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
HVY73_RS01640	353512..354270	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12625.53 Da
• Isoelectric Point: 5.1769

>T165202 WP_064148407.1 NZ_CP057313:349830-350156 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFS

Nucleotide

• Length: 327 bp

>T165202 NZ_CP073764:c2385989-2385693 [Methylovirgula sp. HY1]
ATGATCGTGAGCTTTCGGGACGAGTGGCTCCGCGCGTTCTTTGTCGAGGACACGCCGTCGCGGCACATCCCGTCCGATCT
CGAAAGTCGGCTCTTCCGCAAGCTCCAAATGATCGACGACGCGACGACCGATCAGGATTTGCGGGTGCCGCTCAGCAATC
ACTTCGAAAAGTTGCGCGGCAGTCTTGCGGGTTTCCACTCGATCCGCGTCAACAGCCGATGGCGGCTGGTCTTCCAGTGG
AACGGCAGCCGCGGCGAGGCAGACGGCGTCTATCTCGACGATCACAGTTACAGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10809.60 Da
• Isoelectric Point: 10.1465

>AT165202 WP_064148406.1 NZ_CP057313:350177-350476 [Klebsiella pneumoniae]
MATLKELMAKHSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT165202 NZ_CP073764:c2385684-2385376 [Methylovirgula sp. HY1]
ATGCTGACCACGAAGCGCAAGCCGGCTACGATCGGCGAGATCCTAATCGAGGAGTTCATGCAGCCGATGGGGCTGACCCA
GGCCGCCTTGGCGGAGGCAATGGGCGTTCAGCGCAAGCATGTCAACGAGCTGTGCAACAACCGCCGCAACGTGACGGCGG
CGACCGCGCTCATCCTCGCGCGGGTGTTCGGTAACAGCCCCGACTTCTGGCTGAACGTGCAGCGGCGCAGTGACCTGTGG
GAGGCGATGAACAGTCCAAAGGAGCGCGAGCGGATCGAACGCGCGAAGCCATTGGGCGCGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A6G4MDN2