Detailed information of TA system TA165197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4035689..4036368
• Replicon: chromosome
• Accession: NZ_CP057311
• Organism: Escherichia fergusonii strain RHB30-C07

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: HVY75_RS19475
• Protein ID: WP_002431667.1
• Coordinates: 4035689..4035991 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVY75_RS19480
• Protein ID: WP_000806441.1
• Coordinates: 4036027..4036368 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY75_RS19450	4031065..4032717	+	1653	WP_181203208.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HVY75_RS19455	4032755..4033258	-	504	WP_000667001.1	hypothetical protein	-
HVY75_RS19460	4033255..4034055	-	801	WP_000439795.1	hypothetical protein	-
HVY75_RS19465	4034079..4034558	-	480	WP_181203209.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HVY75_RS19470	4034762..4035556	-	795	WP_000365145.1	TraB/GumN family protein	-
HVY75_RS19475	4035689..4035991	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVY75_RS19480	4036027..4036368	+	342	WP_000806441.1	HigA family addiction module antidote protein	Antitoxin
HVY75_RS19485	4036482..4038986	-	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
HVY75_RS19490	4039293..4040582	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T165197 WP_002431667.1 NZ_CP057311:4035689-4035991 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T165197 NZ_CP073754:2324705-2325124 [Methylomonas paludis]
ATGAATTACCTTTTGGATACCTGTGTCTTATCCGAATTAATAAAACCACAGCCCAACCAGAATGTCGCCACCTGGATTGA
AAAAATTCAGGAGTGCCATTTGTACATCAGCGCTCTGACTATGGGCGAAATTCAAAAGGGAATCTCTAGACTGGAGAATT
CAAAAAAGAAACAATCACTCCAAATTTGGCTGGAACAAGATTTGGCCGCCCGATTTTCGGGCAGAATCTTGTCTGTAGAT
GCTGAAATCTCACTTGAATGGGGAAACATGCAAGGACAAGCGATTCGCTCAGGAACGACATTACCAGTCATAGATAGCCT
GATCGCAGCAACCGCAAAATGTCATAACTTGACGCTTGTAACAAGAAATTTGGAAGACTTCAAAAATCTGCCGATCAATT
TGAATAATCCATGGCTATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT165197 WP_000806441.1 NZ_CP057311:4036027-4036368 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT165197 NZ_CP073754:2324463-2324708 [Methylomonas paludis]
ATGCATTGGCAATTACAAGAAGCAAAAAACAAGCTGAGCCAGGTCATCCACGAGGCACAAACCACAGGTCCACAGACCAT
AACCGTACGCGGAAAAGAGACCGCAGTTGTAATTTCGGCTGCAGACTATCAAAAATTGACAGATAAAAAAGACTCATTAC
TCAAATTCTTCCAGGAATCACCCTTGACTGATGTAGAGCTTGATTTGACGCGATCCAAGGAGAGTGGCAGAGATATCGAG
CTATGA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8