Detailed information of TA system TA165192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3439966..3440765
• Replicon: chromosome
• Accession: NZ_CP057311
• Organism: Escherichia fergusonii strain RHB30-C07

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: HVY75_RS16590
• Protein ID: WP_000347270.1
• Coordinates: 3439966..3440430 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: HVY75_RS16595
• Protein ID: WP_104919918.1
• Coordinates: 3440430..3440765 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY75_RS16560	3434967..3435401	-	435	WP_032303830.1	PTS sugar transporter subunit IIA	-
HVY75_RS16565	3435419..3436297	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HVY75_RS16570	3436287..3437066	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HVY75_RS16575	3437077..3437550	-	474	WP_032303829.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HVY75_RS16580	3437573..3438853	-	1281	WP_181203097.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HVY75_RS16585	3439102..3439911	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HVY75_RS16590	3439966..3440430	-	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HVY75_RS16595	3440430..3440765	-	336	WP_104919918.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HVY75_RS16600	3440914..3442485	-	1572	WP_181668631.1	galactarate dehydratase	-
HVY75_RS16605	3442956..3443726	+	771	WP_001058567.1	2-dehydro-3-deoxyglucarate aldolase	-
HVY75_RS16610	3443751..3444641	+	891	WP_000178100.1	2-hydroxy-3-oxopropionate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T165192 WP_000347270.1 NZ_CP057311:c3440430-3439966 [Escherichia fergusonii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T165192 NZ_CP073754:c1427307-1427026 [Methylomonas paludis]
GTGCTCAAATTACCTTCAGGTATTCTGGCTGACTACATTCATTTGACTGATTTAATTCAACATCATGGCACTGATTTACG
TATGCCGCATTCGCGGGCGATGGGGGATGGTTTATTTGAATTACGGCCAAAAGGGCCGGAAGGTATAGGCCGGGTATTTT
ACTGTATGCAACTGGGTTGCACGGTAGTGATATTGCACAGCTTTATCAAAAAAACCAATACCACGCCGGTTGCCGATTTA
AAACTTGCCAAAAAACGGTTAAAAGAGGTTAAAAATGCATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12349.93 Da
• Isoelectric Point: 4.5392

>AT165192 WP_104919918.1 NZ_CP057311:c3440765-3440430 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGRKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT165192 NZ_CP073754:c1427033-1426722 [Methylomonas paludis]
ATGCATAACGACGAACATTATCAACCTGTACCACACGATGCCGCATTTAGAGCACAGTTGTTGGCGAATCCAGAGGTACA
AGCAGCTTTTCAGGCCAACGCAGCTGAATACGCCATACTCGATGAAATGCTCAATGCCCGCAAAGAAGCCGGACTGACTC
AAGCGCAAGTCGCTGAGCGCATGGGCACCAAAGCGCCAGCCGTAGCCAGATTGGAAAACTCCCTGGCTTCCGGCAAACAT
TCACCATCGATTGATACCTTGCAGAAATATGCCAAAGCCTTAGGCAAAAAGCTGGAAGTGCGTTTGGTATAA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90