Detailed information of TA system TA165104

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73345..73609
• Replicon: plasmid pRHB32-C05_3
• Accession: NZ_CP057245
• Organism: Escherichia fergusonii strain RHB32-C05

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: HVY96_RS24055
• Protein ID: WP_024179514.1
• Coordinates: 73457..73609 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	73345..73407 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY96_RS24040	68584..70875	-	2292	WP_001289276.1	hypothetical protein	-
HVY96_RS24045	70868..71938	-	1071	WP_001542508.1	IncI1-type conjugal transfer protein TrbB	-
HVY96_RS24050	71957..73165	-	1209	WP_175237471.1	IncI1-type conjugal transfer protein TrbA	-
-	73345..73407	-	63	NuclAT_0	-	Antitoxin
-	73345..73407	-	63	NuclAT_0	-	Antitoxin
-	73345..73407	-	63	NuclAT_0	-	Antitoxin
-	73345..73407	-	63	NuclAT_0	-	Antitoxin
HVY96_RS24055	73457..73609	+	153	WP_024179514.1	Hok/Gef family protein	Toxin
HVY96_RS24060	73681..73932	-	252	WP_001291965.1	hypothetical protein	-
HVY96_RS24065	74975..75184	-	210	WP_015059315.1	hemolysin expression modulator Hha	-
HVY96_RS24070	75282..75896	-	615	WP_015059316.1	plasmid IncI1-type surface exclusion protein ExcA	-
HVY96_RS24075	75972..78140	-	2169	WP_181667536.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..116001	116001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5795.12 Da
• Isoelectric Point: 8.7948

>T165104 WP_024179514.1 NZ_CP057245:73457-73609 [Escherichia fergusonii]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T165104 NZ_CP073704:c2138981-2138496 [Burkholderia cenocepacia]
ATGTCCGAACCGCTGCGGCGTGAGCCGAACGCGGCGGTGATCGAGTGGCTCGATGCGCAGAAAGTCGAGACGCTGTATCT
CGCGGCGATCGGTCTCGCGGAATTGCGGTCCGGCGTGGCTGCATTGCCGGAGGGCGCAGGCGGGATTGGCTGCAGCAGAG
TATCGGGCAGCGTGTGCTGTCGCTGTTTCGCGGTCGGATCCTGCCGTTCGACGAGGCGGCGAGCAGGGCGTGCGAGCCTT
TGCGCGCGGACGCGTGCCGCGGGCAACGCGATCGCGGTCGCCGATGGCTACATCGCCGCGACGGCCGAGGCAAACGGCTC
GATCGTGGCCACACGCGACGTCGCGCCGTTCCAGGCGTTCGGGCCGCGCGTCATCGATCCATGCGCCGTCCGGTTCGCGA
TCGCCGGTTCTTTTGGCGTGCCGAAATGCAAAACGCGCCGTGCGTGGCGGCGCGTTTTGAATCGAACCATCAGGCTCGTT
CCCTGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT165104 NZ_CP057245:c73407-73345 [Escherichia fergusonii]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin