Detailed information of TA system TA165096

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 115504..115927
• Replicon: plasmid pRHB32-C05_2
• Accession: NZ_CP057244
• Organism: Escherichia fergusonii strain RHB32-C05

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A8D9PPD8
• Locus tag: HVY96_RS23435
• Protein ID: WP_000775236.1
• Coordinates: 115504..115665 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	115711..115927 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY96_RS23405	110542..110769	-	228	WP_072039828.1	conjugal transfer relaxosome protein TraY	-
HVY96_RS23410	110907..111578	-	672	WP_040100314.1	conjugal transfer transcriptional regulator TraJ	-
HVY96_RS23415	111773..112156	-	384	WP_040100321.1	relaxosome protein TraM	-
HVY96_RS23420	112479..113081	+	603	WP_072156291.1	transglycosylase SLT domain-containing protein	-
HVY96_RS23425	113378..114199	-	822	WP_042973148.1	DUF945 domain-containing protein	-
HVY96_RS23430	114318..114605	-	288	WP_021569031.1	hypothetical protein	-
HVY96_RS23435	115504..115665	-	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	115711..115927	-	217	NuclAT_0	-	Antitoxin
-	115711..115927	-	217	NuclAT_0	-	Antitoxin
-	115711..115927	-	217	NuclAT_0	-	Antitoxin
-	115711..115927	-	217	NuclAT_0	-	Antitoxin
HVY96_RS23440	115939..116658	-	720	WP_042974070.1	plasmid SOS inhibition protein A	-
HVY96_RS23445	116655..117089	-	435	WP_032235533.1	conjugation system SOS inhibitor PsiB	-
HVY96_RS23450	117144..119102	-	1959	WP_181670298.1	ParB/RepB/Spo0J family partition protein	-
HVY96_RS23455	119168..119401	-	234	WP_000005990.1	DUF905 family protein	-
HVY96_RS23460	119464..120006	-	543	WP_042974074.1	single-stranded DNA-binding protein	-
HVY96_RS23465	120032..120238	-	207	WP_077782986.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD / pefC / pefA	1..147532	147532

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T165096 WP_000775236.1 NZ_CP057244:c115665-115504 [Escherichia fergusonii]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEIRLKDGYREVTASMAYESGGK

Nucleotide

• Length: 162 bp

>T165096 NZ_CP073702:c3670575-3670174 [Burkholderia cenocepacia]
ATGCCGCGCTTCATGCTCGACACCAACATGTGTATCTATCTGATGAAAAATCAGCCTGAGCAGGTCGCGAGACGGTTTGC
GCAGTGCTACACGGGCGACGTCGTCATGTCGGCGATCACCTATGCGGAGCTCGAGTACGGCCTGACCGCTTGTGCGAATC
CCGCGCGGGAGCGCCGCCATCTCGCCGCATTGATCGAGGATATTCCCGTCGCGCCTTTCGACGCGGCGGCCGCGCAAGCG
TATGGCCCGGTTCGCGAGGCCACCCGCGAGCGGAAGAAGGATCACCTCGACAAACTGATTGCCGCGCATGCGGTGTCGCT
CGATGTCGTCCTTGTCACCAACAACGAGCGCGATTTCGTCAGCTATCCGGGATTGCGACTGGAGAACTGGCTGAACGACT
AG

Antitoxin

Nucleotide

• Length: 217 bp

>AT165096 NZ_CP057244:c115927-115711 [Escherichia fergusonii]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGCGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin