Detailed information of TA system TA165080

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2064984..2065209
• Replicon: chromosome
• Accession: NZ_CP057243
• Organism: Escherichia fergusonii strain RHB32-C05

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: HVY96_RS09885
• Protein ID: WP_061353447.1
• Coordinates: 2064997..2065209 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2064984..2065042 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY96_RS09840	2060115..2061224	+	1110	WP_072291759.1	DNA-binding protein	-
HVY96_RS09845	2061270..2062064	+	795	WP_061353445.1	DUF1627 domain-containing protein	-
HVY96_RS09850	2062080..2062484	+	405	WP_040073350.1	DUF977 family protein	-
HVY96_RS09855	2062509..2062811	+	303	WP_021522699.1	hypothetical protein	-
HVY96_RS09860	2062813..2063169	+	357	WP_000403785.1	hypothetical protein	-
HVY96_RS09865	2063265..2063672	+	408	WP_061353446.1	hypothetical protein	-
HVY96_RS09870	2063674..2063892	+	219	WP_040073347.1	DUF4014 family protein	-
HVY96_RS09875	2063894..2064259	+	366	WP_001229296.1	HNH endonuclease	-
HVY96_RS09880	2064256..2064600	+	345	WP_040073345.1	hypothetical protein	-
-	2064984..2065042	-	59	-	-	Antitoxin
HVY96_RS09885	2064997..2065209	+	213	WP_061353447.1	type I toxin-antitoxin system Hok family toxin	Toxin
HVY96_RS09890	2065462..2065629	+	168	WP_163426341.1	hypothetical protein	-
HVY96_RS09895	2065696..2065968	+	273	WP_061353448.1	hypothetical protein	-
HVY96_RS09900	2065970..2067028	+	1059	WP_061353449.1	DUF968 domain-containing protein	-
HVY96_RS09905	2067029..2067406	+	378	WP_032215179.1	RusA family crossover junction endodeoxyribonuclease	-
HVY96_RS09910	2067399..2067782	+	384	WP_001345623.1	antitermination protein	-
HVY96_RS09915	2068056..2068541	+	486	Protein_1928	ORF6N domain-containing protein	-
HVY96_RS09920	2068767..2069510	+	744	WP_001345622.1	AraC family transcriptional regulator	-
HVY96_RS09925	2069690..2070082	+	393	WP_001294582.1	holin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2047007..2150009	103002

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7949.53 Da
• Isoelectric Point: 10.0620

>T165080 WP_061353447.1 NZ_CP057243:2064997-2065209 [Escherichia fergusonii]
MLHKRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYESEE

Nucleotide

• Length: 213 bp

>T165080 NZ_CP057243:2064997-2065209 [Escherichia fergusonii]
ATGCTACACAAACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAATCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT165080 NZ_CP057243:c2065042-2064984 [Escherichia fergusonii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTTTGTGTAGCATAGATTGGGCCTCA

Structures

Antitoxin