Detailed information of TA system TA165053

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75251..75505
• Replicon: plasmid pRHB32-C09_2
• Accession: NZ_CP057239
• Organism: Escherichia fergusonii strain RHB32-C09

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HVZ00_RS22590
• Protein ID: WP_181654380.1
• Coordinates: 75251..75457 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	75444..75505 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ00_RS22560	71633..71914	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HVZ00_RS22565	71911..72180	-	270	WP_000079945.1	hypothetical protein	-
HVZ00_RS22570	73094..73951	-	858	WP_019842754.1	incFII family plasmid replication initiator RepA	-
HVZ00_RS22575	73944..74426	-	483	WP_001443034.1	hypothetical protein	-
HVZ00_RS22580	74419..74493	-	75	WP_001442103.1	RepA leader peptide Tap	-
HVZ00_RS22585	74727..74984	-	258	WP_000083834.1	replication regulatory protein RepA	-
HVZ00_RS22590	75251..75457	-	207	WP_181654380.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	75444..75505	+	62	NuclAT_1	-	Antitoxin
-	75444..75505	+	62	NuclAT_1	-	Antitoxin
-	75444..75505	+	62	NuclAT_1	-	Antitoxin
-	75444..75505	+	62	NuclAT_1	-	Antitoxin
HVZ00_RS22595	75801..76112	+	312	WP_000802277.1	hypothetical protein	-
HVZ00_RS22600	76623..76835	-	213	WP_042047803.1	hypothetical protein	-
HVZ00_RS22605	76970..77530	-	561	WP_000139310.1	fertility inhibition protein FinO	-
HVZ00_RS22610	77585..78331	-	747	WP_001541899.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyC	1..145940	145940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7820.35 Da
• Isoelectric Point: 8.8807

>T165053 WP_181654380.1 NZ_CP057239:c75457-75251 [Escherichia fergusonii]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCVTVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T165053 NZ_CP073668:c88042-87611 [Burkholderia contaminans]
ATGACCTACCTGCTCGACGTCAACGTCCTTATCGCCTTGCTGGATCCTGCTCATGTTCAGCACGAACCGGCGCATGCATG
GTTTGGCCGGGTAGGGTATGCCGCATGGTCGACTTGCCCTCTGACTGAGAACGGCGTGTTGCGGATTATCGGCAATCCGA
AGTATCCGAACACGATGGGGTCGCCGGCCTCCGTCGCCCCGCTCGTCGCGCAACTGCGGGCGCACGCCGGGCACACGTTC
TGGCCGGATGATGTGAGCTTGCTGGACGAGAAGCACGTCGACGCCACGCGCTTACTGTCGTCGGCTCAGTTGACCGATAC
GTACTTGCTGGCGCTGGCGCGCGTACACAAGGGCAAGCTGGCGACCTTCGATCGGCGCCTGGTTGTCGACGCCGTGCCGA
ATGGCGCCAAGCATCTCGAACTGATTTCCTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT165053 NZ_CP057239:75444-75505 [Escherichia fergusonii]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin