Detailed information of TA system TA165026

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 115622..116045
• Replicon: plasmid pRHB32-C16_2
• Accession: NZ_CP057230
• Organism: Escherichia fergusonii strain RHB32-C16

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: HVZ03_RS22820
• Protein ID: WP_000775238.1
• Coordinates: 115622..115783 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	115829..116045 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ03_RS22795	111125..111946	-	822	Protein_113	DUF945 domain-containing protein	-
HVZ03_RS22800	112065..112352	-	288	WP_000107535.1	hypothetical protein	-
HVZ03_RS22805	112377..112583	-	207	WP_032248717.1	hypothetical protein	-
HVZ03_RS22810	113554..115221	+	1668	WP_052991863.1	group II intron reverse transcriptase/maturase	-
HVZ03_RS22815	115362..115529	-	168	WP_001422689.1	hypothetical protein	-
HVZ03_RS22820	115622..115783	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	115829..116045	-	217	NuclAT_0	-	Antitoxin
-	115829..116045	-	217	NuclAT_0	-	Antitoxin
-	115829..116045	-	217	NuclAT_0	-	Antitoxin
-	115829..116045	-	217	NuclAT_0	-	Antitoxin
HVZ03_RS22825	116057..116731	-	675	WP_112042691.1	plasmid SOS inhibition protein A	-
HVZ03_RS22830	116728..117162	-	435	WP_011666470.1	conjugation system SOS inhibitor PsiB	-
HVZ03_RS22835	117217..119196	-	1980	WP_112042690.1	ParB/RepB/Spo0J family partition protein	-
HVZ03_RS22840	119262..119495	-	234	WP_000006002.1	DUF905 family protein	-
HVZ03_RS22845	119553..120074	-	522	WP_134887421.1	single-stranded DNA-binding protein	-
HVZ03_RS22850	120555..120871	+	317	Protein_124	pilus assembly protein	-
HVZ03_RS22855	120850..121041	-	192	WP_025269849.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyC	1..145940	145940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T165026 WP_000775238.1 NZ_CP057230:c115783-115622 [Escherichia fergusonii]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T165026 NZ_CP073638:773408-774739 [Burkholderia cepacia]
ATGGCCGCGCGTGCCCGCCACGACCGGCTCGACCTGTGGATGAACGGCATCCCGGTCGGCTACTGGGAAGTCCGGCGCGG
CGTCGAGCGCCTCGTCTACCTTCCGGGCTGGCTCGACGATCCCCAAGGGCGGCCACTGTCGCTGTCGCTGCCCTTCACGC
CCGGCAACCAGCCGCACCAGGGTGCGATCGTTGCCGACTACTTCGACAACCTGCTGCCCGACAGCGAGCCGATCCGTCGC
CGCATCGCGCAGCGCTACCGGCTCGGGTCGACGGCGCCGTTCGAACTGCTCGCGTCGATCGGCCGCGATTGCGTCGGCGC
GATCCAGATGCTGCCGCCCGACGAGACGCCGGTTGACCTCGAGGCGATCGAGGGCGTAACGCTCGACGACGCAGCCGTCG
CCGACGTACTGCGTCACGCGACTGCCGCGCCACTGCCCGGCCGCGCGGAGCCTGACGTCGACCTGCGCCTGTCGATCGCC
GGCGCGCAGGAAAAGACCGCGCTGCTGCGTCAGCGGAACCGCTGGTTGCGGCCGTCGGGCAGCACGCCGACGACGCACAT
CTTCAAGCTGCCGCTCGGGCGCGTCGGGAACATGCAGGCCGACATGCGGACGTCCGTCGAGAACGAATGGCTGTGCTCGA
AACTGGTCGCCGCATATGGCCTGCCGGTCGCGCCGTGCGAGATCGGCAGGTTCGACGACCAGAAGGCACTGATCGTCGAG
CGCTTCGATCGGCGTCCTTCGCGTGACGGCTCGTGGATCCTGCGGCTGCCGCAGGAGGACCTGTGCCAGGCGACCGGCAC
GCCGTCGGGCGCGAAATACGAATCCGACGGCGGCCCTGGCATCGACACGATCATGGGCATCCTCGCGAATTCGGCCGACG
CCGCGCAAGACAGGAGGAATTTCTTCGTCGCGCAGCTCGTGTTCTGGGTGCTGGCGGCGATCGATGGCCACGCGAAGAAC
TTCAGCATCGCGCACCTGCCCGGCAACACGTACCGCAGCACGCCGCTGTACGACGTGCTGTCCGCGCATCCGATCATCGG
CACGCGCCGCAACCAGCTCCCGCCGCGTCGCGCGCGGCTCGCGATGGCCGTGTGCGGGAAGAACCGGCACTACGTGATCG
GCGAGATCCAGCCGCGTCACTGGATCGCGCAGGGCCGTCGCGTCGGCCTGACCGAAGACGACGTGCACGCCGCGATGGCG
GCCGTCGCCGCGCGCACGGAGCCCGCGATTGCCGAAGTCACCGCGCAGATCCCGGCGGATTTTCCGGCGGATGTGGCGGA
CACGATCTTCGACGGCATGCGTCGCCAGAGTCGCAAGCTGGCAGGTGGGTGA

Antitoxin

Nucleotide

• Length: 217 bp

>AT165026 NZ_CP057230:c116045-115829 [Escherichia fergusonii]
TCACACGGATTTCCCGAGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGCGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAAGA
CAAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin