Detailed information of TA system TA165021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3989421..3990100
• Replicon: chromosome
• Accession: NZ_CP057229
• Organism: Escherichia fergusonii strain RHB32-C16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: HVZ03_RS19250
• Protein ID: WP_002431667.1
• Coordinates: 3989421..3989723 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVZ03_RS19255
• Protein ID: WP_000806440.1
• Coordinates: 3989759..3990100 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ03_RS19225	3984797..3986449	+	1653	WP_181668258.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HVZ03_RS19230	3986487..3986990	-	504	WP_181668259.1	hypothetical protein	-
HVZ03_RS19235	3986987..3987787	-	801	WP_181668260.1	hypothetical protein	-
HVZ03_RS19240	3987811..3988290	-	480	WP_181668261.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HVZ03_RS19245	3988494..3989288	-	795	WP_000365145.1	TraB/GumN family protein	-
HVZ03_RS19250	3989421..3989723	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVZ03_RS19255	3989759..3990100	+	342	WP_000806440.1	HigA family addiction module antidote protein	Antitoxin
HVZ03_RS19260	3990214..3992718	-	2505	WP_181668262.1	copper-exporting P-type ATPase CopA	-
HVZ03_RS19265	3993025..3994314	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T165021 WP_002431667.1 NZ_CP057229:3989421-3989723 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T165021 NZ_CP073637:c1903979-1903617 [Burkholderia cepacia]
ATGAAAATCAGCGAACTGGCGGAATTCGACGGCTCGAAGTACCTGAAGGACGAAGAGGCGATTCGTCACTACCTGGCGCA
AGCCTTCGAAGATGGCGATCCGCGGCTGATCCAGGCCGCGCTCGGGAATGTCGCGAAAGCGCGCGGCATGACCGCGCTGG
CGCGCGAGTCCGGCGTAAAGCGCGAAGCGCTTTACCGTGCGCTGTCGGAAGGTGGGAATGCGGAGTTTGCAACGATCATG
AAGGTCGTCGGTGCGTTGGGATTGCACCTGACCGTTGCACCGACCGTGCAGGCGGCAGAAGCGGCACCGGTGCCGGCACC
GACACGCTCGCGCCCGCGTGCCCGCGCTGCGGCGCACACGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13157.08 Da
• Isoelectric Point: 5.7737

>AT165021 WP_000806440.1 NZ_CP057229:3989759-3990100 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT165021 NZ_CP073637:c1904278-1903982 [Burkholderia cepacia]
ATGTTCAAAGTCCTGACGACCCCTCAATTTGATAACTGGCTCGATGGGCTTCGCGACCCGGTTGGCAGCGCGGCAATCAG
CCTGCGCATCGAGCGGGCGAAGCTCGGCAACCTCGGCCAGTGGCGCGCAGTCGGTGACGGCGTTAACGAAATGAAGATCG
ACGTAGGGCCGGGATACCGTGCCTACTTCGTGCGGCGCGGAAAAATCATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGGCGGCGAAGCAAATCGCCGGCGAACTGGAGGATTGA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8