Detailed information of TA system TA164997

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 115124..115545
• Replicon: plasmid pRHB32-C22_2
• Accession: NZ_CP057221
• Organism: Escherichia fergusonii strain RHB32-C22

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: HVZ07_RS22655
• Protein ID: WP_001302184.1
• Coordinates: 115124..115282 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	115347..115545 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVZ07_RS22620	110210..110449	-	240	WP_042357785.1	conjugal transfer relaxosome protein TraY	-
HVZ07_RS22625	110557..111204	-	648	WP_096040777.1	transcriptional regulator TraJ family protein	-
HVZ07_RS22630	111396..111779	-	384	WP_001151530.1	relaxosome protein TraM	-
HVZ07_RS22635	112055..112702	+	648	WP_000601039.1	transglycosylase SLT domain-containing protein	-
HVZ07_RS22640	112998..113819	-	822	WP_001234475.1	DUF945 domain-containing protein	-
HVZ07_RS22645	113938..114225	-	288	WP_000107542.1	hypothetical protein	-
HVZ07_RS22650	114250..114456	-	207	WP_000547965.1	hypothetical protein	-
HVZ07_RS22655	115124..115282	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	115347..115545	-	199	NuclAT_0	-	Antitoxin
-	115347..115545	-	199	NuclAT_0	-	Antitoxin
-	115347..115545	-	199	NuclAT_0	-	Antitoxin
-	115347..115545	-	199	NuclAT_0	-	Antitoxin
HVZ07_RS22660	115557..116276	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
HVZ07_RS22665	116273..116707	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
HVZ07_RS22670	116762..118720	-	1959	WP_000117171.1	ParB/RepB/Spo0J family partition protein	-
HVZ07_RS22675	118786..119019	-	234	WP_000005990.1	DUF905 family protein	-
HVZ07_RS22680	119076..119615	-	540	WP_000290799.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyC	1..147240	147240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T164997 WP_001302184.1 NZ_CP057221:c115282-115124 [Escherichia fergusonii]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T164997 NZ_CP073627:2360450-2360767 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT164997 NZ_CP057221:c115545-115347 [Escherichia fergusonii]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin